MR医学影像文献Yang-2016-MR molecular imaging of tumours usin.pdfVIP

Eur Radiol (2016) 26:4089–4097 DOI 10.1007/s00330-016-4259-9 MOLECULAR IMAGING MR molecular imaging of tumours using ferritin heavy chain reporter gene expression mediated by the hTERT promoter Yan Yang 1,2 Ming-fu Gong 1 Hua Yang 1 Song Zhang 1 Guang-xian Wang 1 Tong-sheng Su 1 Li Wen 1 Dong Zhang 1 Received: 2 June 2015 /Revised: 6 December 2015 /Accepted: 28 January 2016 /Published online: 9 March 2016 # The Author(s) 2016. This article is published with open access at S Abstract Conclusion Reporter gene expression for MRI, using the Fth Objectives Using the human telomerase reverse transcriptase reporter and the hTERT promoter, may be a useful strategy for (hTERT) promoter and the modified ferritin heavy chain (Fth) the non-invasive diagnosis of many types of cancer. reporter gene, reporter gene expression for MRI was exam- Key points ined in telomerase positive and negative tumour cells and • Modified heavy chain of ferritin can serve as an MR reporter xenografts. gene Methods Activity of the reporter gene expression vector • hTERT promoter can direct the expression of reporter gene Lenti-hTERT-Fth1-3FLAG-Puro was compared to constitu- in cancer cells tive CMV-driven expression and to the untransfected parental • MR reporter imaging mediated by hTERT promoter can be control in five tumour cell lines: A549, SKOV3, 293T, U2OS used for cancer diagnosis and HPDLF. In vitro, transfected cells were evaluated for FLAG-tagged protein expression, iron accumulation and Keywords Magnetic resonance imaging . Neoplasm . transverse relaxation. In vivo, tumours transduced by hTERT . Reporter gene expression . Ferritin heavy chain lentiviral vector injection