MR医学影像文献Zeng-2016-Histological validation of cardiac m.pdfVIP

ORIGINAL RESEARCH Histological Validation of Cardiac Magnetic Resonance T1 Mapping for Detecting Diffuse Myocardial Fibrosis in Diabetic Rabbits Mu Zeng, MD,1 Nan Zhang, MD,1 Yi He, MD,1 Zhaoying Wen, MD,1 Zhanhong Wang, MD,1 Yike Zhao, MD,1 Andreas Greiser, PhD,2 Jing An, PhD,3 3 4 5 1 Tianjing Zhang, PhD, Bin Jing, PhD, Xin Zhang, PhD, Zhanming Fan, MD, * and Debiao Li, PhD6 Purpose: To pathologically verify the correlation between native T1 mapping, postcontrast T1 mapping, and extracellu- lar volume fraction (ECV) and myocardial diffuse fibrosis, as determined by collagen volume fraction (CVF). Materials and Methods: Thirty New Zealand white rabbits were randomly divided into the control group (n 5 6), diabe- tes 3 months group (n 5 8), diabetes 6 months group (n 5 8), and diabetes 9 months group (n 5 8). All the rabbits underwent clinical 3.0T magnetic resonance (MR) examinations with pre- and postcontrast modified Look–Locker inver- sion recovery T1 mapping. For the histological study, each rabbit was sacrificed after MR scanning, hematoxylin and eosin and Masson staining of the left ventricular myocardium were performed, and CVF was calculated. Pre- and post- contrast T1 values and ECV were compared to CVF using Pearson’s correlation coefficients. Results: Two rabbits died in each diabetes group, thus each group included six rabbits. ECV calculated from pre- and postcontrast T1 values showed a very strong correlation with CVF (r 5 0.876, P 0.001), whereas postcontrast T1 values exhibited a moderate correlation