致病性大肠杆菌现状分析及检测技术研究进展-生物技术通报.pdfVIP

生物技术通报 ·技术与方法· BIOTECHNOLOGY BULLETIN ( ) 2016, 32 11 :80-92 致病性大肠杆菌现状分析及检测技术研究进展 1 1 1，2 1，2 卫昱君  王紫婷  徐瑗聪   许文涛 （1. 中国农业大学食品科学与营养工程学院，北京 100083 ；2. 农业部转基因生物食用安全监督检验测试中心，北京 100083） 摘 要 ： 致病性大肠杆菌是一类常见的致病菌，能够通过多种感染渠道导致大肠杆菌感染疫情大规模爆发，其耐药性的出 现引起广泛担忧的同时，也促进了新型抑菌方式的研究。 目前大肠杆菌风险毒力因子作为致病的直接因素，备受国内外研究人员 的关注。大肠杆菌检测技术也相应获得了较快的发展，相较传统方法，新型的病原菌检测技术能够快速、特异、准确地检测目标菌。 结合国内外研究现状从大肠杆菌的污染情况、感染途径及症状、耐药性、风险毒力因子及检测方法等方面综述了大肠杆菌毒力评 估及检测技术研究进展。 关键词 ： 致病性大肠杆菌 ；现状分析 ；检测技术 DOI ：10.13560/ki.biotech.bull.1985.2016.11.011 Current Situation Analysis and Detection Techniques of Pathogenic Escherichia coli 1 1 1，2 1，2 WEI Yu-jun WANG Zi-ting XU Yuan-cong XU Wen-tao （1.College of Food Science Nutritional Engineering ，China Agricultural University ，Beijing 100083 ；2. The Supervision ，Inspection and Testing Center of Genetically Modified Organisms ，Ministry of Agriculture of P. R. China ，Beijing 100083 ） Abstract: Pathogenic Escherichia coli is one of the most common pathogenic microorganisms. This kind of pathogen spreads through a variety of ways and its infection has caused many food safety accidents in the world. Though the emergence of its antibacterial resistance has raised concern，it also facilitates institutions and researchers to design new bacteriostatic methods. As direct pathogenic factor，E. coli risk virulence factor has drawn much attention by researchers. Also the technology of detecting E. coli developed rapidly too. Compared to traditional