小麦tawrky41基因克隆及建立在gateway技术上的-华北农学报.pdfVIP

华北农学报 2011, 26 ( 2) : 17-22 TaWRK Y46-1 Gateway 1 1 1 1 1 2 1 1 李 明, 丁 博, 牛有雄 , 吕芳芳 , 杨文丽, Silin Zhong, 孙守钧 , 谢晓东 ( 1. , 300384; 2. BoyceTho pson Institute forPlant Research, CornellUniversity, NY14853, USA) : 158, NCBITaWRK Y46, RT-PCR RNA 870 bp cDNA, NCBITaWRK Y46 , , WRKYGQKWRKYGEK, TaWRK Y46 , TaWRK Y46-1 Gateway,, TaWRK Y46-1 : ;WRKY; Gateway; : Q785; S512. 1035. 3 : A : 1000- 7091( 2011) 02- 0017- 06 Cloning ofWheatTaWRK Y46-1 Gene and Construction ofPlant Inducible ExpressionVector byGatewayCloningTechnique 1 1 1 1 1 LIM ing, DING Bo, NIU You-xiong, LU Fang-fang, YANGW en-li, 2 1 1 Silin Zhong, SUN Shou-jun, XIE Xiao-dong ( 1. Tianjin AgriculturalUniversity, Tianjin 300384, China; 2. BoyceTho pson Institute for PlantResearch, CornellUniversity,USA 14853) Abstract: In this study, thewheatvariety, Yang ai 158(Triticum aestivem L. ), was used as the experi ental a- terial for cloning thewheat TaWRK Y46 gene. Pri ers were designed based on a known wheat TaWRK Y46 gene se- quence in NCBI, subsequently an 870 bp cDNA frag entwas a plified fro totalRNA ofYan ai 158 by RT-PCR. Sequence analysis revealed that the cloned frag ent contains the fulL-length cDNA and is highly ho ologous to the TaWRKY46 sequence inNCBI. The sequence align ent revealed aQ toE changew ithin the conserved WRKYGQK do ain, suggesting itwas anew allele ofTaWRKY46. Hencewe designated it asTaWRK Y46-1. In order to elucidate the functional role of the new Yang ai 158TaWRK Y46-1 allele, a plant inducible over-expression vector, pKIGW- TaWRKY46-1, was constructed viaGa