应用illumina高通量测序技术探究长根菇多糖对小鼠肠道菌群的影响.pdfVIP

222 2015, Vol.36, No.19 食品科学 ※营养卫生 应用Illumina高通量测序技术探究长根菇多糖对 小鼠肠道菌群的影响 王 雪，赵龙玉，赵凤春，张圣方，杨正友* （山东农业大学生命科学学院，山东省农业微生物重点实验室，山东泰安 271018 ） 摘  要：本研究采用Illumina 高通量测序技术检测长根菇多糖对昆明小鼠肠道菌群的影响。利用盐酸林可霉素灌 胃建立肠道微生态失调小鼠模型。将60 只小鼠随机分为5 组：空白对照组、自然恢复组、长根菇多糖低剂量组、 长根菇多糖中剂量组和长根菇多糖高剂量组。无菌条件下采集小鼠盲肠内容物，运用Illumina高通量测序技术分 析各组小鼠盲肠内容物中菌群的结构。结果表明：空白对照组小鼠肠道菌群物种多样性最高，长根菇多糖各剂量 组次之，自然恢复组最低；另外，各组小鼠肠道有益菌与致病菌比例差异显著，长根菇多糖各剂量组有益菌群相 对比例高于自然恢复组，而自然恢复组小鼠肠道中致病菌比例最高。长根菇多糖对小鼠的肠道菌群失调具有恢复 和改善作用。 关键词：长根菇；多糖；肠道菌群；高通量测序 Effect of Oudemansiella radicate Polysaccharide on Intestinal Microbiota in Mice Evaluated by Illumina High-Throughput Sequencing * WANG Xue, ZHAO Longyu, ZHAO Fengchun, ZHANG Shengfang, YANG Zhengyou (Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China) Abstract: The effect of Oudemansiella radicate polysaccharide (ORP) on the intestinal microbiota in Kunming male mice was studied by Illumina high-throughput sequencing in this investigation. The mouse model of intestinal dysbiosis was established by intragastric administration of lincomycin hydrochloride. Sixty mice were randomly divided into five groups including normal control group, natural recovery group and three groups treated with Oudemansiella radicate polysaccharide at different doses. Cecal contents were collected aseptically, and the intestinal microbiota was detected by illumina high- throughput sequencing. The results showed that the diversity and abundance of intestinal bacteria were varied in the mice in the five groups. Among these, the no