补肾养血方对卵巢早衰大鼠中抗苗勒氏管激素amh-中国试验动物学报.docVIP

补肾养血方对卵巢早衰大鼠的影响(傅金英,王冰玉,胡俊攀? 【摘要】目的 建立雷公藤多苷致卵巢早衰大鼠的模型，研究补肾养血方对卵巢早衰大鼠中抗苗勒管素（Anti-Mullerian hormone, AMH）的影响。 方法 使用雷公藤多苷建立卵巢早衰大鼠模型，造模成功后，分别采用高，中，低剂量补肾养血方对大鼠灌胃，每日一次，共30天。给药完毕后于显微镜下观察各组大鼠卵巢组织形态。酶联免疫吸附法、免疫组化染色法和定量反转录聚合酶连锁反应（qRT-PCR）分别检测大鼠血清中AMH含量以及卵巢内AMH的蛋白和mRNA表达水平。结果 组织形态学显示高中低剂量组的卵泡和黄体的生长均比模型组好；酶联免疫吸附法、免疫组化和qRT-PCR结果显示补肾养血方呈剂量依赖性逆转雷公藤多苷导致大鼠血清中AMH浓度以及卵巢中AMH 表达水平的降低。 结论 补肾养血方可逆转雷公藤多苷引起卵巢中AMH水平降低，提示补肾养血方具有防止卵巢早衰的功效。 【关键词】补肾养血方; 卵巢早衰; AMH Effect of bushenyangxue prescription on the levels of anti-mullerian hormone in rat with premature ovarian failure JI Xia*, FU Jin-ying, WANG Bing-yu, HU Jun-pan (Department of obstetrics and gynecology, Henan province hospital of TCM, Zhengzhou 450002, China) [Abstract] Objective Establish premature ovarian failure (POF) model in female Sprague Dawley by tripterygium wilfordii, and investigate the effect of bushenyangxue prescription on the levels of anti-mullerian hormone (AMH). Method After POF model was established, rats were gave by gavage of different dosage of Bushenyangxue prescription for 30 days. The changes of histomorphology on rat ovarian tissue were observed by hematoxylin-eosin staining. Serum AMH concentraction, protein and mRNA expression of AMH were measure with ELISA, immunohistochemical staining and qRT-PCR, respectively. Results The follicle and corpus luteum were atrophied after tripterygium wilfordii challenge, which was improved after treatment with Bushenyangxue prescription. Serum AMH, protein and mRNA expression of AMH were decreased tripterygium wilfordii-treated rats; this decrease was inhibited after treatment with Bushenyangxue prescription. Conclusion Our study indicates that Bushenyangxue prescription could preserve the AMH levels of POF rats. These findings suggest that Bushenyangxue prescription may be a useful strategy to treat POF. [Key words] bushenyangxue prescription; premature ovarian failure (POF); AMH 卵巢早衰（premature ovarian failure，POF）是一种多因素导致的卵巢功能提前减退的症状，指女性在40岁以前出现持续闭经，不育，伴随雌激素降低，卵泡雌激素（fol