拟南芥低叶绿素荧光lcf3基因的克隆与功能分析-作物学报.pdfVIP

拟南芥低叶绿素荧光lcf3基因的克隆与功能分析-作物学报.pdf

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作物学报 ACTA AGRONOMICA SINICA 2016, 42(5): 690695 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2016.00690 拟南芥低叶绿素荧光LCF3 基因的克隆与功能分析 刘凌云 刘 浩 赵 晶 王艳霞 王棚涛 / / , 475004 : , EMS lcf3-1 (lower chlorophyll fluorescence 3-1)lcf3-1 LCF3 PsbW , LCF3 T-DNA LCF3 Fv/Fm , LCF3 , LCF3 PsbW PSII-LHCII : ; ; ; Map-based Cloning and Functional Analysis of Low Chlorophyll Fluorescence Gene LCF3 in Arabidopsis thaliana * LIU Ling-Yun, LIU Hao, ZHAO Jing, WANG Yan-Xia, and WANG Peng-Tao Henan Key Laboratory of Plant Stress Biology / State Key Laboratory of Cotton Biology / College of Life Sciences, Henan University, Kaifeng 475004, China Abstract: Chlorophyll fluorescence has exclusive role for determining the kinetics of light energy absorption, transmission, dis- sipation and distribution in leaf photosynthesis. In this study, a mutant was screened by chlorophyll fluorescence equipment from EMS mutagenesis of wild-type Arabidopsis Col-0, which showed lower F /F than wild-type and was named lcf3-1 (lower chlo- v m rophyll fluorescence 3-1). Genetic analysis of lcf3-1 mutant suggested that the mutation was controlled by single recessive gene. LCF3 gene, which encodes PsbW protein, was isolated by map-based cloning. The T-DNA insertion mutant lcf3-2 showed low chlorophyll fluorescence phenotype as lcf3-1, and this phenotype could be complemented by LCF3 gene of wild-type. These ex- peri

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