Bmi-1对干性相关分子转录调控的初步研究-第三军医大学学报.DOC

Bmi-1调节干性（Stemness）相关分子转录的初步分析 阮 艳1，蹇 锐1，程小星2，牛翰婕2，周恒宇3，郑宏庭3，胡福泉3）第三军医大学1基础医学部微生物学教研室，重庆400038；3新桥医院内分泌科，重庆 4000372解放军总医院309临床部，北京 100091 摘 要：目的Bmi-1对转录的。方法构建Bmi-1过表达载体pCI-GFP-bmi1和pCI-GFP-hbmi1；瞬时转染ES细胞CCE和人神经胶质瘤细胞U87；半定量RT-PCR检测、、c-yc、lf4和ox2及肿瘤细胞中CD133、Nestin等干性分子的表达结果Bmi-1过表达的表达，、ox2和lf4的表达无明显变化结论Bmi-1参与了对肿瘤及胚胎干细胞中干性相关分子的转录调节。 关键词：Bmi-1；；；转录调控 中图法分类号：Q2; Q7; R394 文献标识码：A Transcriptional Regulatory Effects of Bmi-1 on Stemness Associated Genes Ruan Yan1, Jian Rui1, Cheng Xiao-Xing 2, Zhou Heng-Yu 3, Zheng Hong-Ting3 （1Department of Microbiology, Third Military Medical University, Chongqing 400038, China; 2PLA General Hospital, 309th Clinical Division, Beijing 100091, China; 3Department of Endocrinology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China） Abstract: Objective To investigate the transcriptional regulatory effects of Bmi-1 on stemness-associated genes in cancer and embryonic stem cells. Methods: Eukaryotic expression vector pCI-GFP-mbmi and pCI-GFP-hbmi was constructed and transiently transfected into CCE and U87 cells respectively. Semi-quantitative RT-PCR was used to detect mRNA expression of stemness factors in ES cells and tumor cells. Clone formation essay was used to elucidate the potential to form self-renewal clones of CCE. Results Overexpression of Bmi-1 in CCE up-regulated the expression of Nanog. The expression of other stemness factors such as Oct-4, Sox2 and Klf4 had no significant change. U87 cells transiently transfected with Bmi-1 promoted the expression of Oct-4 and Nestin. The expression level of c-Myc was reduced in the two cell lines. Overexpression of Bmi-1 promoted the formation of self-renewal clones in CCE cells. Conclusion Bmi-1 was involved in the transcriptional regulation of stemness-associated genes in cancer and embryonic stem cells . Key words: Bmi-1; Stem cells; Stemness associated genes; Transcriptional regulation 肿瘤干细胞（Cancer Stem Cells, CSCs）是肿瘤组织