多囊卵巢综合征大鼠骨骼肌细胞胰岛素受体底物1及其丝氨酸307位点.pdfVIP

中国组织工程研究 第 16 卷 第33 期 2012 –08 –12 出版 Chinese Journal of Tissue Engineering Research August 12, 2012 Vol.16, No.33 多囊卵巢综合征大鼠骨骼肌细胞胰岛素受体底物1及其丝氨酸307位点 磷酸化的表达*★ 安广丽，王 娈，马瑞欣 Expression of insulin receptor substrate 1 and its serine 307 site phosphorylation in rat skeletal muscle with polycystic ovary syndrome An Guang-li, Wang Luan, Ma Rui-xin 文章亮点： Department of 多囊卵巢综合征大鼠骨骼肌细胞中胰岛素受体底物 1 蛋白表达下调及其丝氨酸307 位点磷酸化蛋白表达上调 Endocrinology, Affiliated Hospital of 与多囊卵巢综合征大鼠骨骼肌胰岛素抵抗的发生密切相关。 Qingdao University Medical College, Qingdao 266003, Abstract Shandong Province, China BACKGROUND: Increased serine 307 phosphorylation of insulin receptor substrate 1 is involved in the occurrence of insulin resistance in the skeletal muscle. An Guang-li★, Studying for master’s OBJECTIVE: To observe the expression of insulin receptor substrate 1 and its serine 307 phosphorylation in the skeletal degree, Department muscle of polycystic ovary syndrome rats. of Endocrinology, Affiliated Hospital of METHODS: Female Sprague-Dawley rats were randomized to model group and control group. Insulin and human Qingdao University Medical College, chorionic gonadotrophin were administered to the model group rats which were fed with high fat diet and 50 g/L glucose Qingdao 266003, solution. The rats in the control group were given normal diet and injected normal saline daily. Shandong Province, China RESULTS AND CONCLUSION: Compared with the control group, expression of insulin receptor substrate 1 decreased angul-0707@ 163.com significantly (P 0.05), while its serine 307 phosphorylation increased obviously (P 0.05) in the model group at 6 weeks after intervention. These findings suggest that reduction of insulin receptor substrate1 protein expression and