快速溶剂萃取仪提取葡萄籽中原花青素条件优化.pdfVIP

※工艺技术 食品科学 2013, Vol.34, No.14 157 快速溶剂萃取仪提取葡萄籽中原花青素条件优化 * 袁 琳，袁春龙 ，胡立志，张世杰，杨晓雁，杨 健 (西北农林科技大学葡萄酒学院，陕西 杨凌 7 12100) 摘  要：利用快速溶剂萃取仪优化提取新品种‘媚丽’葡萄籽中原花青素。结果表明：利用响应面优化方法，得到 最佳萃取条件为提取时间13min 、提取压强116 1psi 、甲醇体积分数80%、提取温度80 ℃，此时原花青素提取率的理 论预测值为6.817%，重复提取3次，测得提取率为6.286%，相对误差为7.79%，纯度4 1.02%。 关键词：葡萄籽原花青素；快速溶剂萃取；Box-Behnken试验设计 Optimization of Extraction Conditions for Proanthocyanidins from Grape Seeds by Accelerated Solvent Extraction * YUAN Lin ，YUAN Chun-long ，HU Li-zhi ，ZHANG Shi-j ie ，YANG Xiao-yan ，YANG Jian (College of Enology, Northwest A F University, Yangling 7 12100, China) Abstract ：Response surface methodology was applied to optimize the extraction of proanthocyanidins from seeds of the new grape cultivar “Meili” using an accelerated solvent extraction (ASE) system. The optimal extraction conditions were found to be 13 min, 1161 psi, 80% and 80 ℃for extraction time, pressure, methanol concentration and temperature, respectively. The maximum predicted extraction yield of proanthocyanidins under the optimized conditions was 6.817% indicating a relative error of 7.79% as compared with the actual value of 6.286% obtained after the third repeated extraction. The purity of proanthocyanidins under the optimized conditions was 4 1.02%. Key words ：proanthocyanidins (PCs) ；ASE ；Box-Behnken experimental design 中图分类号：TS255.36 文献标志码：A 文章编号：1002-6630(2013)14-0157-06 doi:10.7506/spkx 1002-6630-20 1314032 ‘媚丽’葡萄是一种抗病性强、综合性状优良的 减少，这使得单个样品的提取费用也显著降低。本实验 酿酒与鲜食兼用的中熟品种，适宜在我国华北、西北地 研究目的在于利用加速溶剂萃取仪优化葡萄籽的提取条 区及南方适宜地区推广。原花青素(proanthocyanidins ， 件，以期为以后研究提供一些借鉴。 PC) ，也叫缩合单宁，是由儿茶素C4～C8或C4～C6位连 接组成的聚合物。原花青素是葡萄多酚的重要组成，大