板蓝根中红细胞凝集效应组分的谱效关系研究.PDFVIP

中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 1 期 2012 年 1 月 ·125 · 板蓝根中红细胞凝集效应组分的谱效关系研究 1 2 1 1 1 3* 孙 琴 ，马 丽 ，李 兰 ，胡晓燕 ，姜 黎 ，肖小河 1. 泸州医学院药学院，四川 泸州 646000 2. 首都医科大学中医药学院，北京 100069 3. 解放军 302 医院全军中药研究所，北京 100039 摘 要：目的 研究板蓝根的红细胞凝集活性成分。方法 对板蓝根药材进行系统化学部位分离，采用红细胞凝集活性检测 法探讨各化学部位的抗病毒活性；针对活性最强的部位建立 10 批不同产地样品的 HPLC 指纹图谱并进行生物测定，运用数 理统计方法研究谱效相关性。结果 板蓝根正丁醇萃取部位对家兔红细胞的凝集作用最强；谱效相关性研究发现 2 号、11 号共有峰（保留时间分别为 7.23、43.00 min ）的相对峰面积所占比例越大，样品的凝集作用也越强。结论 2 号、11 号峰可 能为板蓝根抗病毒作用的特征峰，所建立的板蓝根 HPLC 特征图谱与药效作用的关系，可为建立“指纹图谱与药效关联” 的中药质量控制模式提供理论依据和数据支持。 关键词：板蓝根；凝集活性；指纹图谱；谱效关系；抗病毒活性 中图分类号：R284.2 ；286.02 文献标志码：A 文章编号：0253 - 2670(2012)01 - 0125 - 06 Spectrum-activity relationship of hemagglutination components in Isatidis Radix 1 2 1 1 1 3 SUN Qin , MA Li , LI Lan , HU Xiao-yan , JIANG Li , XIAO Xiao-he 1. Department of Pharmacy, Luzhou Medical College, Luzhou 646000, China 2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China 3. PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China Abstract : Objective To study the active components in Isatidis Radix with agglutination effect on red blood cells. Methods The different extracts from Isatidis Radix were obtained by the means of systematic solvent extraction and their antivirus activity was studied by hemagglutination test. The HPLC fingerprints of the most active extracts from Isatidis Radix were established and also the extracts were bioassayed. The spectrum-activity relationship of the extracts was studied by mathematical statistics method. Results The n-butanol extracts had the best agglutination effect on red blood cells of the rabbit. The study on the spectrum-activ