胆汁性肝硬化大鼠肠系膜组织内皮素系统的变化-F6Publishing.DOCVIP

Name of journal: World Journal of Gastroenterology ESPS Manuscript NO: 882 Columns: BRIEF ARTICLES Increased endothelin receptor B and G protein coupled kinase-2 in mesentery of portal hypertensive rats Du QH et al. Mechanisms of portal hypertension Qing-Hong Du, Lin Han, Jun-Jie Jiang, Peng-Tao Li, Xin-Yue Wang, Xu Jia Qing-Hong Du, Lin Han, Jun-Jie Jiang, Peng-Tao Li, Xin-Yue Wang, Xu Jia, Department of Pathology, School of Fundamental Medicine, Beijing University of Chinese Medicine, Beijing 100029, China Author contributions: Li PT proposed the study; Li PT and Du QH performed research and wrote the first draft; Han L collected and analyzed the data; All authors contributed to the design and interpretation of the study and to further drafts; Li PT is the guarantor. Supported by A grant from National Key New Drug Creation Project of China, No. 2009ZX09102 Correspondence to: Dr. Peng-Tao Li, Department of Pathology, School of Fundamental Medicine, Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, Beijing 100029, China. lipengtao0413@ Telephone: +86-10 Fax: +86-10Received: October 23, 2012 Revised: January 25, 2013 Accepted: February 5, 2013 Published online: Abstract AIM: To elucidate the mechanisms of mesenteric vasodilation in Portal hypertension (PHT), with a focus on endothelin signaling. METHODS: PHT was induced in rats by common bile duct ligation (CBDL). Portal pressure was measured by directly via catheters placed in portal vein tract. The level of endothelin-1 (ET-1) in the mesenteric circulation was determined by radioimmunoassay, and the expression of the endothelin A receptor (ETAR) and endothelin B receptor (ETBR) were assessed by immunofluorescence and Western blot. Additionally, expression of G protein coupled kinase-2 (GRK2) and β-arrestin 2, which influence endothelin receptor sensitivity, were also studied by Western blot. RESULTS: PP of CBDL rats increased significantly (11.89 ± 1.38 mmHg vs