荧光脂质微泡的制备及其在兔肝脏显影中的评价-第三军医大学学报.DOCVIP

亚微米级荧光脂质微泡的制备及其肝脏成像的实验研究 乔璐1, 高文宏1 , 张莉1, 梁红敏2, 李露1，朱梅2*,李佩倞1， 刘政1* (1.第三军医大学新桥医院超声科，重庆 400037；2.昆明医学院第一附属医院超声科，云南 昆明 650032) 摘要 目的:制备荧光标记的脂质微泡,检测一般物理性质、荧光标记情况以及评价在兔肝内的显影效果。方法: 用二甲基亚砜（DMSO）溶解细胞膜绿色荧光分子探针(DiO)后，抽取少量溶解液加至脂质冻干粉内与溶媒液相溶，全氟丙烷置换瓶内空气，最后机械振荡仪高速振荡制成带有荧光标记的脂质微泡,检测其微泡的一般物理性质、荧光标记情况；经兔耳缘静脉团注荧光微泡后动态观察微泡在兔肝实质的显影效果，手术切取部分肝脏，冰冻切片后在荧光显微镜下观察荧光素在肝脏内的分布情况。结果: 荧光标记的脂质微泡形态呈圆形，大小均一，微泡浓度范围（4 —7)×109/ ml,微泡平均粒径为（429 ± 119）nm，激光共聚焦显微镜下微泡外壳被绿色光圈环绕。兔肝脏造影可见持续饱满的增强显影效果，荧光显微镜下观察到肝脏内荧光素面积分布较少。结论:荧光标记的脂质微泡大小均一,粒径大小达到进入肺循环标准，在兔肝脏内显影效果显著。 关键词 DiO标记物; 脂质微泡; 超声; 肝脏 中图法分类号：R445.1；R-331;R-332 Preparation of DiO-labeled fluorescent microbubbles and assessment of its liver enhancement Qiao Lu 1, Gao Wenhong1 ，Zhang Li1, Liang Hongmin2, Li Lu1 , ZHu Mei2*Liu Zheng1* (1.Department of Ultrasound, Xinqiao HospitalThird Military Medical University, Chongqing 400037,China?; 2. Department of Ultrasound, the First Affiliated Hospital of Kunming Medical University,Kunming 650032,China） Abstract Objective To prepare fluorescent microbubbles and evaluate its enhancement in rabbit liver. Methods Fluorescence lipids were prepared by lyopyilization and then mixed with Dimethyl sulfoxide (DMSO) dissolved DiO. The DiO-labeled microbubbles were made by high speed mechanical agitation with a RPM of 5500. The particle size of submicron fluorescent lipid microbubbles (SFLM) were detected by Particle Coulter and were observed DiO-labeled level by confocal laser scanning microscope. The liver contrast imaging was acquired by intravenous injection of the microbubble at 0.02 ml/kg. Results The mean concentration of SFLM was (4- 7)×109/ml and the mean particle size was (429 ± 119)nm. The contrast enhanced ultrasound using SFLMs was significant in rabbit liver. Conclusion SFLMs that are stable ultrasound contrast agents, of well-distributed shape and size have been developed and deserve further exploration as targeted drug delivery. Key words DiO; lipid microbubbles; ultrasound; liver Corresponding auth