转外源凝集素基因棉花对棉蚜的抗性鉴定.pdfVIP

Cotton Science 2005, 17( 2) :72~ 7 转外源凝集素基因棉花对棉蚜的抗性鉴定 1 1 1 1 1 1 2 , , , , , , ( 1. 江苏省农业科学院经济作物研究所, 南京210014; 2. 江苏省农业科学院植物保护研究所, 南京210014) : 转外源凝集素基因棉花工 植株在蚜虫发生期稳定地表现出抗蚜性状, 转化 一代群体 抗感植株分离比例符合3B 1, 抗蚜基因是以单 一位点整合到棉花染色体组中经过4 个世 代的抗蚜鉴定自交纯合和选择, 获得遗传组成纯合的转基因抗蚜虫棉花新品系对3 个转外 源凝集素基因棉花品系2 个形态抗蚜品种( 系) 和6 个常规品种( 系) 进行抗蚜性鉴定结果 表明, 3 个转外源凝集素基因品系对棉蚜的抗性均达到高抗水平; 川抗77 在生育期内均中抗 棉蚜, 川棉109 在苗蚜期和恢复期感蚜, 而在伏蚜期中抗棉蚜; 6 个常规品种( 系) 皆高感棉蚜 利用三种接蚜处理对不同类型抗蚜品种( 系) 进行鉴定的结果相同, 繁殖行间自然传播蚜虫省 略人工接蚜环节, 是开展转基因棉花抗蚜性鉴定的 一种简便高效的方法 : 棉花; 转基因; 外源凝集素基因; 抗蚜鉴定 : S435. 622 : A : 1002-7 07( 2005) 02-0072-07 Identification of Aphis ( Ap his g ossy p ii Glover. ) Resistant of Cotton ( Gos- syp ium hirsut um L. ) with Trans- exogenous Agglutinin Gene 1 1 1 1 1 1 XIAO Song-hua , LIU Jian-guang , WU Qiao-juan , DI Jia-chun , XU Na-i yin , CHEN Xu-sheng , 2 BAI Li-xin ( 1. Institute of Industrial Crop s, J iangsu A cademy of A gricultural Sciences, N anj ing 210014, China; 2. Institute of Plant Protection, Jiangsu A cademy of A gricultural Sciences, N anj ing 210014, China ) Abstract During aphis occurring period, aphid- resistant character can express stably in engineering plants of trans-exogenous agglutinin gene cotton. T he segregation proportion of the first transformed generation population accords w ith 3 B 1, the aphis-resistant gene is integrated into cotton genome w ith single locus. N ew varieties of transgenic cotton resistant to aphis can be obtained by successive w ith identification, selfing and selection through four generations. With the experimental materials of three cotton varieties w ith trans-exogeno