雄激素对体外成骨细胞OPG及其配体基因表达的影响-上海申远医学.PDFVIP

　2007 2 13 2 　Chin J Osteoporos, February 2007,Vol 13, No.2 77 ·· OPG 张川　李孝鹏　符诗聪　邹志强　朱雅萍　张风华 :　, OPG OPGL mRNA , 、。 　 , ; 10-10 mol L、 10-9 mol L、10-8 mol L 3 ;RNA, RT-PCR OPG OPGL mRNA 。 　 , OPG , OPGL OPG 。 　 OPG OPGL 。 :;;(OPG);(OPGL); Regulation of estrogen receptor expression by androgens (Histomorphometry)in osteoblastic cells　ZHANG Chuan, LIXiaopeng, FUShicong, et al.Shanghai Institute of Traumatology and rthopaedics, Medical School of ShanghaiJiaotong University　200025, China Abstract:Objective　To in estigate the effects of androgen on the expression of OPG and OPGL, and to further clarify the mechanism of regulation of androgens on osteoblast in the process of inducing osteoclast differentiation and acti ation.Methods 　The second-passage osteoblasts that were tested to be characterized as being of osteoblastic phenotype were used in experiments;androgen were de ided into 3 groups of 10-10 mol L、10-9 mol L、 10-8 mol L and then exert influence on osteoblasts;mRNA expressions of OPG and OPGL were semi-quantified by RT-PCR.Results　No cell toxicity was found in all concentrations we used in experiments.The expression of OPG in osteoblasts was significiantly increased and up-regulated by androgen, and androgen promoted a decrease in the ratio of OPGL and OPG with time.Conclusion　Androgen can regulate the expression of OPG and OPGL mRNA in gene-le el in osteoblasts. Key words:Androgen;Osteoblast;OPG;OPGL;Gene expression 　　(OPG System) , , OPG (OPG )(OPG , Ligand, ODF, RANKL, TRANCE)mRNA [1] , OPG , 、。 。 、 1　 。 , 1.1　 。 1.1.1　 DMEM (GIBCO ), Ⅱ :(03BK26) (SIGMA ), (D- :200025　, Hanks), (), (、、、、); ()