植物根系构型原位观测识别技术研究进展-生态学杂志.pdfVIP

生态学杂志ChineseJournal of Ecology摇 2011,30(9):2066-2071 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 植植物根系构型原位观测识别技术研究进展* ** 李克新摇 宋文龙 摇 朱良宽 (东北林业大学机电工程学院,哈尔滨 150040) 摘摇 要摇 根系是植物从土壤中获取养分和水分并输送给植物体的重要器官,由于土壤的不 透明属性阻碍了根系的观测,缺乏有效的原位根系观测方法已经构成了对植物根系深入研 究的技术瓶颈。 本文首先从根系研究的技术需求出发,对破坏性根系研究法、非破坏性根 系二维观测法(微根管法)和根系三维构型原位观测方法如X 射线计算机层析成像(X鄄ray computed tomography,XCT)、核磁共振成像法(magnetic resonance imaging,MRI)等进行了 综述,并总结了各自的优缺点。 其次,重点对XCT 和MRI 法进行了比较,有研究表明,MRI 法不适用于根系三维构型的观测。 指出自然土壤介质下,XCT是根系三维构型定量化分析 未来的发展趋势。 关键词摇 植物根系;原位观测;X 射线计算机层析成像;微根管 中图分类号摇 TP274摇 文献标识码摇 A摇 文章编号摇 1000-4890(2011)9-2066-06 Observation and measurement of plant root architecture in situ:A review. LI Ke鄄xin, ** SONGWen鄄long ,ZHULiang鄄kuan (Schoolof MechanicalandElectricalEngineering,North鄄 east Forestry University,Harbin 150040,China). Chinese Journal of Ecology,2011,30(9): 2066-2071. Abstract:Rootsystemistheimportantorganofplantforabsorbingandtransportingnutrientsand water fromsoiltotheplantbody. However,theopaqueattributesof soilhindertheobservationof root system,andthelackof root systemobservationmethodsin situhasbecomethetechnological bottleneck for the in鄄depth study of root system. Inview of thetechnical needsof root systemre鄄 search,thispaper summarizedthedestructiveroot systemresearch method (traditional method), non鄄destructive two鄄dimensional root system observation method (minirhizotron),and three鄄di鄄 mensional root system architecture observation methods in situ,including X鄄ray computed tomo鄄 graphy (XCT) and magnetic resonance imaging (MRI),and generalized their advantages and