水解蛋黄粉改善鼠骨代谢和生长的研究.pdfVIP

※营养卫生 食品科学 2012, Vol.33, No.23 327 1,2 3 3 3 1,* 吴 静 ，宋兴安 ，堀江健二 ，金武祚 ，刘立明 (1.江南大学 食品科学与技术国家重点实验室，江苏 无锡 214122 ； 2.江南大学医药学院，江苏 无锡 214122 ；3. 日本富尔玛株式会社(PFI)，日本 京都 6158245) 摘 要：以小鼠成骨细胞株MC3T3-E1 、大鼠破骨细胞和大鼠为实验模型，通过测定碱性磷酸酶(ALP)活性、抗酒 石酸酸性磷酸酶(TRAP)染色、骨溶蚀陷窝和5-溴脱氧尿嘧啶核苷(BrdU)荧光染色等多种骨代谢生化指标，研究源于 鸡蛋蛋黄的水解蛋黄粉改善骨代谢的生理作用机制。结果表明：水解蛋黄粉通过促进成骨细胞生长、抑制破骨细胞 分化与破坏、提高骨端生长板细胞活性等机制改善骨代谢、促进骨骼生长。 水解蛋黄粉；骨代谢；成骨细胞；破骨细胞 Promoting Effect of Bonepep on Bone Metabolism and Growth 1,2 3 3 3 1,* WU Jing ，SONG Xing-an ，HORIE Kenji ，KIM Mujo ，LIU Li-ming (1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China； 2. School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China ； 3. Pharma Foods International Co. Ltd., Kyoto 6158245, Japan) Abstract ：The purpose of this study was to elucidate the promoting effect of Bonepep, bioactive peptides derived from egg yolk on bone metabolism and growth and its physiological mechanism. For this, mouse osteoblastic MC3T3-E1 cells, isolated rat osteoclast and rats were used and alkaline phosphatase (ALP) activity measurement, TRAP staining, bone erosion lacunae and BrdU staining were carried out in this study. It was found that Bonepep improved bone metabolism and growth through many different mechanisms such as promotion of osteoblast growth, inhibition of osteoclast growth and differentiation and enhancement of bone cell activity. Key words Bonepep bone metabolism osteoblast osteoclast ： ； ； ； 中图分类号： TS201