超高效液相色谱-串联质谱测定苹果制品中的棒曲霉素.pdfVIP

No．1 OF 检验检疫学刊JOURNALINSPECTIONAND 2010年第1期V01．20 QUARANTINE 超高效液相色谱一串联质谱测定苹果制品中的棒曲霉素 黄菲菲1 韩奕奕1 李丹妮2 韩惠雯1 (1．农业部食品质量监督检验测试中心上海200436；2．上海市兽药饲料检测所) 摘要建立了简单、快速测定苹果制品中棒曲霉素的超高效液相色谱一串联质谱确证检测方法。样品 该方法操作方便、快速，灵密度高，可满足苹果制品中棒曲霉素测定的要求。 关键词棒曲霉素；超高效液相色谱一串联质谱；苹果制品 中图分类号0657．63 DeterminationofPatulinin ProductsUltraPerformancePerformance Apple by I-Eigh Mass LiquidChromatography—-TandemSpectrometry Huiwenl Feifeil，HanYiyil，LiDanni2：Han Huang (1．Food and Centerof of Institute QuMit)rSupervisionTesting MinistryAgriculture，Shanghai200436；2．Shanghai of andFeeds VeterinaryDrug Contr01) methodofdeterminationof in Abstract：A patulinappleproductsby(UPLC—MS／MS)was wereextractedwithacetonitrileand muhifunctionalcolumn．and purifiedthroughMycrosep@228AflaPatcleanup detectedUPLC—MS／MS．Themethodhada linear by good relativity(r：O．999)between ratesindifferentsubstrate from83％to RSD10％．Themethodis samplesrange 98％，with forthe of curateandsuitable andconfirmationdeterminationin quantification patulin appleproducts． PerformancePerformance Mass Keywords：Patulin；UltraHigh LiquidChromatography—TandemSpectrometry (UPLC—MS／MS)；Appleproducts l前言 2．1试剂 棒曲霉素(Patulin)，又称展青霉素，是由荨麻青乙酸铵(分析纯，国药集团化学试剂有限公 霉、扩展青霉和棒曲霉等霉菌