微流控芯片2(杨晓涵)应用.ppt

Aptamer-Based Origami Paper Analytical Device for Electrochemical Detection of Adenosine** The operating principle of the sensor. DMM=digital multi-meter. . d) A plot of fluorescence intensity If versus the concentration of adenosine Cadenosine in the sample. e) Sequences of the aptamer strand, the fluorophore strand, and the quencher strand. a,b) Fluorescence micrographs of the oPAD showing 10 mm fluorescent microbeads preloaded in one split channel c) A fluorescence micrograph of a paper fluidic device having two channels preloaded a) Calibration curve for detecting adenosine using the oPAD with and without amplification by a capacitor. The error bars represent standard deviation of readings from a DMM for three independent measurements. b) Sequences of DNA strands of the aptamer sensor. The sequence for binding to adenosine is in italics. Microfluidic immobilization of physiologically active Caenorhabditis elegans Subcellular resolution imaging and optical manipulation of physiologically active C. elegans. (b) Cross-section of the chip showing the immobilization method Microfluidic chip for mechanical immobilization of C. elegans. (a) Diagram of the chip with numbered arrows showing C. elegans manipulation steps. Cross-section of the microfluidic device showing the immobilization region. The device is vertically cut along the line from port-B to port-C. The thermally bonded compression layer and flow layer are visible. A PDMS channel array is molded from the flow-1 layer with 10 μm thickness. A 15- to 20-μm-thick PDMS membrane is formed between the compress-1 and flow-2 layers. This device will be plasma bonded to a cover glass. Microfluidic device integrated with off-chip components. Manual operation of the microfluidic device. The table indicates the state of each port during operation. (a) Loading of the animal from syringe. (b) Immobilization of the animal in linear orientation. (c) Unloading of the animal to the agar plate. Lifespan analysis of t