单克隆抗体技术在小麦贮藏蛋白研究及其遗传改良中的-生物技术通报.pdfVIP

生物技术通报 # # BI OTECHN OLOG Y B ULLETI N 2005 6 * * 李建国 李巧云 郝春燕 蔡民华 晏月明 ( , 100037) : 近20 年来, 人们制备了许多小麦种子贮藏蛋 的单克隆抗体( Monoclonal Antibody, McAb) , 一方 面作为有效工具研究胚乳贮藏蛋 ( 主要是麦谷蛋 聚集体特定的谷蛋 亚基及醇溶蛋 ) 的结构与功能关系; 另一方面用作诊断试剂( diagnost ic tools) , 为筛选具有合适加工品质的小麦品种提供依据本文综述了国内外单 克隆抗体技术在小麦贮藏蛋 研究及其遗传改良中的应用进展, 并展望其应用前景 : 小麦 单克隆抗体 贮藏蛋 ELISA (Enzyme lin ed immunosorbent assay) Application of Monoclonal Antibody Technology in the Studies of Wheat Endosperm Storage Proteins and Genetic Improvement Li Jianguo Li Q iaoyun Hao Chunyan Cai Minhua Yan Yueming ( Co l le ge of L if e S ci ence , Cap it al N ormal Univ ers i ty , B eij in g 100037) Abstract: Dozens of Monoclonal Antibodies have been prepared t o wheat storage proteins in recent tw enty years. Monoclonal Ant ibodies raised to w heat storage proteins appear t o be pow erful t ool in the studies of the rela- tionship betw een st ruct ure and function of w heat endosperm storage proteins (mainly wheat glut en polymer, specific gluten subunits and gliadins) . On the other hand, lots of Monoclonal Antibodies can be used as diagnost ic tools to determ ine the content of specific proteins in seed and flour using enzyme- lin ed immunosorbent assay ( ELISA) . This article has reviewed recent progress in the application of monoclonal antibody technology in the studies of w heat endosperm storage proteins and genet ic breeding . T he prospect of its applicat ion in these studies is also discussed. Key w ords: Wheat Monoclonal antibody Storage proteins ELISA , ( Glutenins)