双孢蘑菇基质降解能力退化的差异蛋白质组学分析.pdfVIP

Mycosystema 菌 物 学 报 15 May 2011, 30(3): 508-513 jwxt@ ISSN1672-6472 CN11-5180Q ©2011 Institute of Microbiology, CAS, all rights reserved. 双孢蘑菇基质降解能力退化的差异蛋白质组学分析 1,2 1 1 1 1 2* 陈美元 王泽生 廖剑华 李洪荣 蔡志欣 谢宝贵 1 福建省农业科学院食用菌研究所 福州 350014 2 福建农林大学菌物研究中心 福州 350002 摘 要：对双孢蘑菇 CGMCC No. 0214 及其基质降解能力退化菌株 0214-3、0214-5 进行了蛋白质双向电泳（2-DE 或2D） 分析，发现了 2 个明显的、可重复的差异蛋白质，在退化菌株中分别为上调和下调表达，且 2 个退化菌株表现一致。根据质 谱（MALDI-TOF/MS ）分析和数据库检索结果，2 个差异蛋白质初步鉴定为肌动蛋白和 NADH 脱氢酶铁硫蛋白 3 。 关键词：食用菌，蛋白质组，双向电泳，差异蛋白质，质谱分析 Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus CHEN Mei-Yuan1, 2 WANG Ze-Sheng1 LIAO Jian-Hua1 LI Hong-Rong1 CAI Zhi-Xin1 2* XIE Bao-Gui 1Edible Fungi Institute of Fujian Academy of Agricultural Sciences, Fuzhou 350014, China 2Mycological Research Center of Fujian Agriculture and Forestry University, Fuzhou 350002, China Abstract: The technique of two-dimensional electrophoresis (2-DE or 2D) was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No. 0214 and its substrate-decomposing ability degenerated strains 0214-3, 0214-5. As a result, two proteins were found to be up- and down-expressed, respectively, with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively. The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A. bisporus .