红掌细菌性疫病病原菌的pcr特异性检测-植物病理学报-中国农业.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA　 42(1):1-9(2012) 红掌细菌性疫病病原菌的PCR特异性检测 1 2 2 2 1* 卢梦玲 , 周晓云 , 曾伟达 , 陈一新 , 刘琼光 1 2 ( 华南农业大学资源环境学院, 广州510642; 广州花卉研究中心, 广州510360) 摘要: 红掌细菌性疫病(Xanthomonas axonopodis pv. dieffenbachiae,简称Xad)是红掌等天南星科花卉毁灭性病害,该病通 过带菌种苗调运不断在我国扩散蔓延,国内尚未有检测方法。 通过筛选和重新设计引物,建立了Xad 的PCR检测方法,结 2 果表明,利用引物Xad-F/ Xad-R 进行PCR,能扩增出检测Xad 的特异性DNA片断,其灵敏度可达1×10 CFU/ mL,DNA 的 最低检出限为0.44 ng/ μL,可用于红掌苗的带菌检测和红掌细菌病害的鉴定。 关键词: 红掌;Xanthomonas axonopodis pv.dieffenbachiae;PCR;检测 Specific PCR detection of Xanthomonas axonopodis pv. dieffenbachiae 　 LU 1 2 2 2 1 1 Meng-ling ,ZHOU Xiao-yun ,ZENG Wei-da ,CHEN Yi-xin ,LIU Qiong-guang 　 ( College of Natural Re- 2 source Environment South China Agricultural University,Guangzhou 510642,China; Guangzhou Flowering Plant Research Centre,Guangzhou 510360,China) Abstract: Bacterial blight ofAnthurium caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad) is a destructive disease. The diseaseisspreadedwithplant seedlingsandnomethodfor thepathogenicbacteria de- tection in our country. In this study,a PCR system for Xad detection with the specific primers Xad-F/ XadR was developed. TheresultsshowedthatXad couldbe detected specificallyby the system andthethresholdsof 2 PCR were 1×10 CFU/ mL for Xad suspension and0.44 ng/ μL for its DNA. The method developed in this study is effective to detect and identify the pathogen inAnthurium plants. Key words:Anthurium andraeanum;Xanthomonas axonopodis pv. dieffenbachiae;PCR;detection 中图分类号: S432.42　 　 　 　 　 文献标识码: A