金毛狗脊中的黄酮类化合物在纳滤膜中的纯化-南京工业大学学报.DOCVIP

超滤-纳滤技术在金毛狗脊黄酮分离纯化中的应用 韩 伟蒋一帅 （华东理工大学 药学院 中药现代化研究中心，上海 200237） 摘要：提取超滤纳滤。在十二烷基硫酸钠的辅助下动态循环提取金毛狗脊超滤。超滤操作压力0.14MPa，温度45℃，溶液pH值6.0，操作时间min，回收率61.3%，截留率18.52%；纳滤操作压力0.4MPa，温度45℃，溶液pH值6.0，操作时间90min，此时料液浓度提高2.19倍，68.29%，截留率53.92%。大孔树脂纯化实验，方法能节省操作时间、提高操作效率同时有效提高浓度关键词：超滤；纳滤；金毛狗脊；黄酮 中图分类号：R284.2 文献标志码：A 文章编号：1671-7627（201）-XXXX-XX Application of ultrafiltration-nanofiltration technology in purification of flavonoids from Cibotium barometz HAN Wei，JIANG Yishuai， （Engineering Center for Traditional Chinese Medicine ModernizationSchool of pharmacy, East China University of Science and Technology, Shanghai 200237, China） Abstract：extraction-ultrafiltration-nanofiltration operation was used to purify the flavonoids in Cibotium barometz. The flavonoids was extracted by the dynamic circulating water solution with the assist of SDS, then the macromolecular impurities were removed by ultrafiltration and the feed liquid was concentrated by nanofiltration membrane finally. The optimal parameters were as follows：ultrafiltration’s pressure 0.14MPa, temperature 45℃, pH of the solution 6.0, operation time 90min, the yield of ultrafiltration 61.30%, the retention rate 18.52%. Nanofiltration’s pressure 0.40MPa, temperature 45℃, pH of the solution 6.00, operation time 90min, the trapped fluid’s flavonoids concentration raised to 2.190 times of the original and the yield reached to 68.29%, the retention rate 53.92%. Comparing the nanofiltration and macroporous resin purification, the result shows this succession operation method was time-saving and efficient, and the flavonoids was concentrated effectively. Key words：Ultrafiltration；Nanofiltration；Cibotium barometz；flavonoids 金毛狗脊（Cibotium barometz）是真蕨亚门蚌壳蕨科植物，为我国民间传统药用植物。中药里常说的狗脊是将金毛狗脊作为入药部位，因此成为国内外专家学者的研究重点。临床实验表明有肝益肾[]、[1]、活血利尿[]和抑制血小板凝聚[3]等，可以用来治疗风湿腰痛[]、骨质疏松[]及小便异常等症状。现代药理研究结果表明，含有山奈素、金粉蕨素、蕨素R、蕨素Y[]等丰富的黄酮类化合物。 [10-12]。操作时间长以去离子水为溶剂，表面活性剂动态循环提取狗脊根茎中的黄酮类物质，超滤纳滤后续工艺 1 实验 1.1 主要材料 金毛狗脊，产地安徽，上海雷允上药品有限公司中国药品生物制品检定所化学纯CP，上海凌峰化学试剂有