Fat and Oil Oxidation by Electron Spin Resonance （ESR） Spin-Trapping Method.pdfVIP

JournalofFoodScienceandEngineering3(2013)584-591 … … N 《 FatandOilOxidationbyElectronSpinResonance(ESR Spin·T·rappingMethod HiromiFujinoandMitsukoUkai HokkaidoUniversityofEducation，Hakodate—shi,Hokkaido040—8567,Japan Received：September9，2013／Published：November20，2013 Abstraet：0xidationoffatsandoilsduringstoragecausestheirdegradationandlossofnutritionalvalueandaDpearance．Electronspin resonance(ESR)istheonlymethodthatcanbeusedtodirectlyobservetheradicals，Inthisstudy，theauthorsusedanESR spin-trappingmethodtostudytheoxidationoftriacy1glycer0ls(TAG)containingdifferentfattyacids(FAs)commonlyfoundinfood． TheESR adductsignalswereanalyzedtostudytheeffectofdoublebondsandthechainlengthoftheFAsofTAG onoxidation． OxidationwasconductedbyapplyingUVirradiationtoTAGbydissolvingitinN-tert-buthyl-c-【phenylnitrone(PBN)，whichtrapped theradicalsinducedintheTAGasanESRadductsigna1．ThedetectionwasclearlysuccessfuI．Therewerenodifferencesinthespectra oftristearin(18：01andtripalmitin(16：0)：thus，itcanbeconcludedthatthelengthofthecarbonchainoftheFAsofTAGdoesnotaffect theoxidationreactions．However，theESRspectraoftristearin(18：0)triolein(18：1)andtrilinolein(18：3)wereclearlydifferentdueto theDresence／absenceofanewpeakcorrespondingtonewinducedradicals．1eadingtotheconclusionthatdoublebondsplayamajor roleintheoxjdationreactionsoffatsandoils． Keywords：ESR，spintrapping，fatsandoils，TAG，oxidation，UV irradiation． 1．IntrOducti0n whichcausestheirdegradationandlOSSofnutritional valueandappearance[4]．Oxidizedfatsandoilscan Alongwithproteinsandcarbohydrates，fatsandoils alsocontributetofoodpoisoning[1]．Therefore，ithas aremajornutrientsrequiredforahealthyhumanbody．