无蹼壁虎gekkoswinhonis卵泡发育的研究.pdfVIP

31 生命科学研究 Vo l . 3 N o. 1 1 9 9 9 3 L if e Science Research M ar . 1999 : 1007 -7847 ( 1999) 01-0058-0 6 X ( Gekko swinhonis) 蔡亚非 　李　君 　陈壁辉 ( 241000) : 用光镜观察了无蹼壁虎卵泡发育过程. 结 显示 其卵泡有显著的季节 性变化 可分两期 即卵黄形成前期及卵黄形成期. 卵泡来源于胚床内的卵母细 胞及其周围的卵泡细胞 其发育过程有4 个主要特点: 1) 卵泡鞘为复层扁平上皮 内层细胞碱性磷酸酶( A L P) 阳性率为100% ; 2) 卵黄形成前期卵泡核出现染色体 及波状核膜; 3) 颗粒层早期由3种细胞构成: 梨状细胞、中间型细胞及小细胞; 4) 后期颗粒层解体 卵母细胞内出现大量的卵黄小板. 最后讨论卵泡鞘A L P 高含 量及卵黄小板形态差异的原因及意义. : 无蹼壁虎; 卵泡发育; 颗粒层; 卵黄小板; 碱性磷酸酶 : Q954 . 4; Q959. 6　　　: A Folliculogenesis in Gekko swinhonis CA I Y a-f ei LI Jun C HEN Bi-hui ( D epar tm ent o f Bio log y Anhu i N or m al U niver sity W uhu 241000 A nhui China) Abstract: T he fo lliculog enesis in Gekko sw inhonis w as ob served by light microscop e. T here w er e clear seasonal changes in t he foll iculogenesis. It could be divided int o t w o st ages: t he pr ev it ellog enesis and vit el logenesis. T he f ollicle st em med fr om t he oo cyt e and foll icul ar cells sur rounding t he oocyt e. It h ad fo ur ch aracterist ics: 1) T he t hecal layer w as str atif ied squamo us epit helium. T he posit ive rate of A LP ( alkal ine phosph atase) of t he cells in t he inner l ay er of t he t hecal layer w as 100% ; 2) T he chrosome and undulat ing membrane came int o being in t he nu cleu s of t he f ollicle in previt ellog enesis; 3) T here w ere t hree dist inct cel l t yp es in t he g ranulosa÷pyrif orm cells interm ediat e cells and small cells ; 4) T he g ranulosa increasing ly disint eg rat ed and the yolk pl at elet came int o being in t he o ocy te in v it ellog ene- sis . T he r eason and meaning of high