光质对灵芝生长及抗氧化酶系统的影响.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 12 期 2011 年 12 月 • 2529 • 光质对灵芝生长及抗氧化酶系统的影响 郝俊江，陈向东，兰 进* 中国医学科学院 北京协和医学院药用植物研究所，北京 100193 摘 要：目的 以飞船搭载的灵芝 Ganoderma lucidum 菌株为材料，研究不同光质对灵芝生长形态及抗氧化酶活性的效应， 找出适于灵芝生长的最佳光质，为光质选择提供理论依据。方法 栽培灵芝时进行不同光质处理，在灵芝不同生长发育时 期观测其形态变化，并测定抗氧化酶体系中主要酶活性。结果 不同光质处理的弹孢前期灵芝菌盖厚度与菌盖表面环纹数 与对照差异显著。不同光质处理对灵芝子实体产量和灵芝孢子粉产量均有影响。红色、蓝色光质处理提高了过氧化物酶 （POD ）、过氧化氢酶（CAT ）活性。蓝色光质处理提高了可溶性蛋白量，并且使丙二醛（MDA ）在生长末期保持相对较 低水平，从而延缓了灵芝子实体衰老。绿色、黄色光质抑制了抗氧化酶活性，导致灵芝子实体可溶性蛋白量下降及 MDA 量上升，加速了灵芝子实体的衰老过程。结论 蓝色光质可以使灵芝子实体维持较高的抗氧化酶水平，促进可溶性蛋白合 成，从而增强灵芝代谢，延缓灵芝衰老。 关键词：灵芝；光质；抗氧化酶；可溶性蛋白；丙二醛 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2011)12 - 2529 - 06 Effect of light on growth in Ganoderma lucidum and anti-oxidative enzyme activities HAO Jun-jiang, CHEN Xiang-dong, LAN Jin Institute of Medicinal Plant Develepment, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China Abstract: Objective In order to provide theoretical basis for cultivation, the effects of different light on the form and anti-oxidative enzyme activities of Ganoderma lucidum ever carried by the first spacecraft of China were studied to find the optimum light for the growth of G. lucidum. Methods G. lucidum was planted under different light. Their forms and activities of anti-oxidant enzymes were observed. Results There were significant differences in the form of the thickness and the ring pattern numbers of the fruit body before ejection. The yield of both fruit bodies and spores were affected under different light. Red and blue light increased the activities of POD and CAT. Blue light incresed the contents of soluble protein and MDA. In contrast, green and yellow light decreased the activities of anti-oxidant enzymes, the contents of soluble protein and MDA. Conclusion These results indicate that blue light could accelerate the growth of G. lucid