软骨多糖对小鼠ca761乳腺癌血管生成的影响 effects of cartilage polysaccharide on angiogenesis of mice ca761 breast cancer cells.pdfVIP

现代食品科技 Modern Food Science and Technology 2009, Vol.25, No.6 软骨多糖对小鼠Ca761乳腺癌血管生成的影响 张国蓉，梁玲玲，付辞 （天津科技大学食品工程与生物技术学院，天津 300457） 摘要: 目的是研究软骨多糖对小鼠Ca761 乳腺癌肺转移的抑制作用及其对血管生成的影响。方法是建立 615 近交系小鼠乳腺癌 肺转移模型，设模型组和治疗组。检测软骨多糖肺转移抑制率、肺部石蜡切片 HE 染色观察肿瘤侵袭情况、免疫组化CD34 观察瘤组 织微血管密度（MVD ）以及VEGF 蛋白表达。结果表明，软骨多糖肺转移抑制率达 44.4%；通过肺部 HE 染色发现治疗组转移情况 明显低于模型组；免疫组化检测结果显示治疗组CD34 蛋白标记的 MVD、VEGF 蛋白表达水平均低于模型组。可见软骨多糖能明显 抑制小鼠 Ca761 乳腺癌的血管生成，从而抑制肿瘤转移，与软骨多糖能够下调瘤组织VEGF 蛋白表达有关。 关键词: 软骨多糖；乳腺癌；血管生成 中图分类号：R332 ；文献标识码：A ；文章篇号:1673-9078(2009)06-0581-03 Effects of Cartilage Polysaccharide on Angiogenesis of Mice Ca761 Breast Cancer cells ZHANG Guo-rong , LIANG Ling-ling, FU Ci （College of Food Engineering Biotechnology, Tianjin University of Science Technology, Tianjin 300457, China ） Abstract: The inhibitory effects of cartilage polysaccharide on angiogenesis of mice Ca761 breast cancer cells in 615 mice were investigated. 615 Mouse models of breast cancer were established. The mice were randomly divided into two groups. Mice in the model group were injected i.p with saline, while the mice in the treatment group were injected i.p. with cartilage polysaccharide. The inhibitory effect of cartilage polysaccharide was analyzed by measuring the pulmonary metastasis inhibition rate. Metastasis of breast cancer cells was analyzed by lung node HE staining. The MVD changes of tumor tissues were detected by CD34 staining in vivo, and the expressions of VEGF protein were determined by immunohistochemical method. Results showed that the pulmonary metastasis inhibition rate was 44.4%. HE staining of tumor metastasis lung node showed that, compared with the model group, the pulmonary metastasis