噬菌斑电子图像的计算机处理及其自动计数.PDFVIP

维普资讯 第 18卷 4期 中 国 病 毒 学 18 (4)：387— 390 2003年 8月 VIR0L0GICA SINIGA August 2003 噬菌斑 电子图像 的计算机处理及其 自动计数 张俊祥 1，肖 茜2，方呈祥 h，范成鹏 ，朱石桥 ，张珞珍 (1．武汉大学生命科学学院，湖北武汉，430072；2．华中科技大学计算机科学与技术学 院，湖北武汉 。430074) PlaqueElectronicImageSegmentandAutomaticCountingbyComputer ZHANGJun—xiang，XIAOQian，FANGCheng—xiang ，FANCheng—peng。， ZHU Shi—qiao ，ZHANG Luo—zhen (1．CollegeofLifeSciences,WuhanUniversity,Wuhan430072，China；2．DepartmentofComputerandTechnology HuazhongUniversiytScienceandTechnology,Wuhan430074，ChinaJ Abstrat：Plaquescounting isa routine method in thehteoryresearch and practicedapplication of microbiology．Becauseofweak contrastbetween plaquenad htebackground ofhteplate，nad hte plaqueslinkingtogether,aseriouserrorwasproducedusingautomaticcountingbycomputer．Sohtat plaqueshavebeencountedbyartificialmethod．Inthepresentstudy，anew mehtodwasestablishedfor automaticcountingplaquesbycomputer．Atfirst，Theplaqueswereformedby infectingEscherichia coliwith 入 bacteriophage，andprepraedashtemethodofsoft—agra layerplate．Th eelectricalimages countedhteplaqueswereobtainedby screeninghteplaquesplates．A representativeraea，inwhichhte plaqueswerelinkedeachohter,waspickedoutfrom eachimage，nadhna dedwiht watershedalgorithm． Th eplaqueslinkingtogehterweresepraatedintoindividualplaquealoneinhteimages．Andhten，hte imageswerecountedwiht htemethodofgrowht algorithm basedonraeabycomputer．Th eexacteffect ofcountingplaqueswasinagreementwiht artificialmethod ．Itindicateshtenew mehtodofcounting plaquescna beusedinautomaticcountingbycomputer． Keywords：Hna dleimage；Watershedalgorihtm；Automaticcounting；Growthalgorithm basedonraea． 摘要：噬菌斑平板计数是微生物学理论研究与实际应用 中常用的方法之一。