Folin_Ciocalteu比色法测定苹果渣中多酚.docVIP

Folin_Ciocalteu比色法测定苹果渣中的多酚 ※分析检测食品科学 2010, Vol. 31, No. 04 179 Folin-Ciocalteu比色法测定苹果渣中的多酚 韩菊，魏福祥 (河北省污染防治生物技术实验室，河北科技大学环境科学与工程学院，河北石家庄 050018) 摘要：采用超临界 CO2萃取技术从苹果渣中提取多酚，以没食子酸为标准物质，系统研究了 Folin-Ciocalteu(FC) 比色法测定多酚的条件，建立了 Folin-Ciocalteu比色法测定苹果渣中多酚的方法。适宜的显色条件为 FC试剂用量 0.3mL、饱和碳酸钠溶液用量 1.5mL、反应时间 20min、反应温度 25～35℃。结果表明：在 1～10μg/mL范围内， 没食子酸吸光度与其质量浓度呈良好的线性关系，r＝ 0.9994；并进行了高、中、低 3个不同加标水平的加标回 收率测定实验，相应的回收率分别为 99.66%、98.79%、98.20%，平均值为 98.82%，RSD为 1.93%。该法测定 样品快速、准确，重复性好，可应用于实际样品的测定。 关键词：多酚； Folin-Ciocalteu法；苹果渣；超临界 CO2萃取 Determination of Polyphenols in Apple Pomace by Folin-Ciocalteu Colorimetry HAN Ju，WEI Fu-xiang (Key Laboratory of Contaminant Controlling in Biology of Hebei Province, College of Environmental Science and Engineering, Hebei University of Science and Technology, Shijiazhuang 050018, China) Abstract：Folin-Ciocalteu colorimetric assay for polyphenols extracted from apple pomace by supercritical CO2 extraction using gallic acid as reference substance was systematically investigated for developing an optimal colorimetric method for the determination of polyphenols in apple pomace. The optimum colorimetric conditions were determined as follows: 0.3 mL of Folin- Ciocalteu reagent, 1.5 mL of saturated Na2CO3 solution, 20 min of reaction time and 25－35 ℃ of reaction temperature. The calibration curve for quantifying polyphenols exhibited a good linearity in the range of 1－10 μg/mL (r = 0.9994). The average recoveries of gallic acid at high, medium and low spike levels were 99.66%, 98.79% and 98.20% (n = 5), respectively, which were reaveraged at 98.82%, with a RSD of 1.93%. The proposed method demonstrates the advantages of rapidity as well as high accuracy and reproducibility. Key words：polyphenols；Folin-Ciocalteu colorimetry；apple pomace；supercritical CO2 extraction 中图分类号： O657.32文献标识码： A文章编号： 1002-6630(2010)04-0179-04 苹果渣是果汁厂榨汁后的下脚料，其中含有丰富的研究上[3-6]，而对苹果多酚测定的系统研究较少。不论是 活性成分多酚类物质。苹果多酚是苹果中所含多元酚类先进的多酚提取技术的探索，高纯度多酚产品的分离、 物质的总称，是苹果