茶树在干旱条件下的mRNA差异表达mRNADifferential-天恩泽.PDF

茶叶科学 2012 ，32 （1）：53~58 Journal of Tea Science 茶树在干旱条件下的 mRNA 差异表达 * 陈盛相，齐桂年 ，夏建冰，邹瑶，单虹丽 四川农业大学园艺学院茶学系，四川 雅安 625014 摘要：采用20%PEG-6000 对福鼎大白茶无性系扦插苗进行模拟干旱处理，应用mRNA 差异显示技术研究干旱 与正常浇水对照的mRNA 差异表达，发现 1 个在干旱条件下减量表达片段N3-5 。序列分析和同源性比对表明， N3-5 与茶树无性系 Sajin 茶叶色突变基因组序列标签 S31.B15 （Accession ：DQ443473.1 ）有75% 的同源性。根 据序列相似同源基因的功能推测，该片段可能与茶树的抗旱机制有关。 关键词：茶树；干旱胁迫；mRNA 差异表达 中图分类号：S571.1 ；Q756 文献标识码：A 文章编号：1000-369X （2012 ）01-053-06 mRNA Differential Expression of Camellia sinensis under Drought Conditions * CHEN Sheng-xiang, QI Gui-nian , XIA Jian-bing, ZOU Yao, SHAN Hong-li Department of tea Science, College of Horticulture, Sichuan Agricultural University, Ya’an 625014, China Abstract: 20% PEG-6000 was used for simulating drought treatment to Camellia sinensis cv. Fuding-dabaicha clone. mRNA differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) was used to study the mRNA differential expression between drought stress and irrigated contro1. One down regulated gene was found under drought stress. Sequence analysis and homology alignment showed that N3-5 showed 75% homogenicity with the Camellia sinensis clone Sajin tea leaf mutant color tag S31.B15 genomic sequence (Accession ：DQ443473.1). It was suggested that the fragments N3-5 was related to drought tolerance mechanism of tea plant according to the functions of their homologous sequences. Keywords: Camellia sinensis, drought stress, mRNA differential expression 我国茶叶主产区在长江中下游一带，每年 sinensis cv. Fuding-dabaicha ）进行干旱胁迫， 夏秋期间，晴日多，气温高，空气湿度低，蒸 通过观察了福鼎大白茶的叶片形态变化，同时 发量大，常有伏旱和秋旱发生，对茶树生长以