哺乳动物基因组印记的保护与维持.PDFVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2016, 38(10): 1276– 1280 DOI: 10.11844/cj cb.2016.10.0095 哺乳动物基因组印记的保护与维持 * * 张美玲 朱屹然 杨树宝 王春凤 栾维民 马 馨 (吉林农业大学动物科学技术学院, 长春130118) 摘要 基因组印记是生殖细胞基因组发生父源或母源单等位基因表达的一种表观调控现象, 哺乳动物单性生殖的胚胎不能存活, 表明在全基因组重编程过程中, 印记的保护和维持十分重要。 因此, 在受精后全基因组发生的主动与被动去甲基化过程中, 必须保留印记位点在配子发生期间获 得的差异甲基化状态。为了更深入地理解植入前胚胎重组期间参与保护和维持基因组印记的分子 机制, 尤其是基于ZFP57(zinc finger protein 57)和TRIM28(tripartite motif-containing 28)相互作用组成 的转录共抑制复合体, 该文阐述了该复合体及其他相关因子近几年的研究进展, 并探讨了这些分子 对基因组印记保护与维持的表观调控机制。 关键词 基因组印记; 重编程; DNA 甲基化 Protection and Maintenance of Genomic Imprints in Mammal Zhang Meiling, Zhu Yiran, Yang Shubao, Wang Chunfeng, Luan Weimin*, Ma Xin* (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China) Abstract Genomic imprinting is an epigenetic regulation phenomenon, which can restricts monoallelic ex- pression to either the maternally or paternally inherited copy of the gene. Uniparental embryos leads to embryonic lethality indicates that protecting and maintaining of imprinting are critical in the process of genome reprogram- ming. Therefore, differential methylation status in imprinted loci which acquired during gametogenesis must be maintained and protected in the process of an active and passive demethylation after fertilization. To further study these molecules, especially the transcriptional co-repressor complex achieved via ZFP57/TRIM28 (zinc finger pro- tein 57/tripartite motif-containing 28) interactions, which protect imprinted methylation sites during preimplanta- tion embryonic development. In this review, the recent study progress of this complex and other related factor (eg. DPPA3, DNMT1) are summarized, and the epigenet