植物激素——油菜素内酯.ppt

* * The CYP85A2 gene incicated *** has a second catalytic activity. * * * * * * * * The data shown on the right include responses of plants treated with increasing amounts of exogenous BL (x axis) and decreasing amounts of light (y axis), with hypocotyl length shown on the z axis. * Transcription mediated by promoters of CPD or CYP85A2 was monitored by fusion to the luciferase reporter gene (LUC). Transcription is controlled by both light and circadian cycles. * Scale bars in a and b are 1 cm. * * * CLV2 and Cf9 are plant proteins involved in developmental and pathogen signaling, whereas Toll is an animal protein involved in immune signaling. These types of receptors are very abundant in plant and animal genomes. The cartoons above the confocal micrographs show the distribution of BKI1-YFP before BL treatment (when it is present at the plasma membrane, as shown by the strong outline of the cells in the micrograph), and 5 or 10 minutes after BL treatment, when it moves into the cytoplasm, as shown by the decreased yellow outline and increase yellow fluorescence within the cells. * * A genetic screen was used to look for genes that partially reverted the bri1 mutant phenotype. In this case, an activation screen was used, so the identified protein is overexpressed. Overexpression of BAK1 partially reverts the bri1 mutant phenotype * * * * * * * * The cartoons show the two halves of the transcription factor (purple) fused independently to the “bait” protein (BRI1 in this case) and the “interactor” (BKI1 here). When the bait and interactor physically associate in the yeast cell, they form a functional transcription factor which in this case permits growth of the yeast cells, indicated as streaks on a dark background. * This image shows a two dimensional gel revealing proteins extracted from BL-treated plants (labeled with Cy5 dye which fluoresces red) or control plants (labeled with Cy3 dye which fluoresces green). When the protein abundance is identical in both sa