基于EST的新基因克隆策略.PDF

HEREDITAS (Beijing) 2008 3 , 30(3): 257―262 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.00257 EST 1 1 2 1 刘媛 , 蔡嘉斌 , 蒋国松 , 童强松 1. , 430022; 2. , 430022 : 表达序列标签(expressed sequence tags, EST) 是从随机选择的 cDNA 克隆进行单向测序获得的短的 cDNA 序列, 代表一个完整基因的一部分。随着生物信息学和基因定位的迅猛发展, EST 已成为基因定位、基因 克隆、基因表达分析的有力工具。近年来, 由于EST 数据库的迅速扩张, 运用EST 来克隆和定位基因, 使得新 基因克隆的策略发生了革命性变革。尽管存在一些不足, 实践证明EST 可大大加速新基因的发现与研究。本文 将就EST 技术尤其是它在新基因克隆中的应用策略作详细介绍。 : 表达序列标签; 基因克隆; 基因 Strategies for cloning of novel genes based on EST 1 1 2 1 LIU Yuan , CAI Jia-Bin , JIANG Guo-Song , TONG Qiang-Song 1. Department of Pediatric Surgery, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China 2. Department of Urology, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China Abstract: Expressed sequence tags (EST) are short, randomly selected single-pass nucleotide sequence reads derived from cDNA libraries and represent a small part of a gene. Along with the development of bioinformatics and genetic localization, EST has already become a powerful tool for mapping, cloning and expression profiling of genes. Recently, because of the fast distension of EST databases, application of EST in gene mapping and cloning leads to revolutionary change in the strategies for cloning of novel genes. Despite of some insufficiencies, it has been proved that EST could promote the dis- covery and research of novel genes. In this article, an introduction about EST, especially EST-based strategies for cloning of novel g