提高埃及糖橙遗传转化效率的研究.pdfVIP

39 4 () Vol.39 No.4 2013 8 Journal of Hunan Agricultural University (Natural Sciences) Aug 2013 DOI:10.3724/SP.J.1238.2013.00371 1,2 1,2 1,2 1,2 1,2 1,2 1,2* ,  (1. 410128 2. 410128)   20 d 10 d (AS) 20 mg/L AS19 pH 5.4~5.7 GUS GUS 29.4% PCR S571.1 A 1007–1032(2013)04–0371–06   Further study on improving genetic transformation efficiency of succari orange HU Wei1,2, YANG Li1,2, XIE Yu-ming1,2, ZHOU Xiao1,2, GE Hong-juan1,2, LI Da-zhi1,2, DENG Zi-niu1,2* (1.College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410128, China; 2.National Center of Citrus Improvement, Changsha Subcenter, Changsha 410128, China)   Abstract: To increase the genetic transformation efficiency of sweet orange infected by Agrobacterium , an explant derived from internode stem segments of sucarri orange seedling was adopted to optimize light regimes and co-cultivation condition for the growth. Meantime, seedless gene was transferred to succari orange. The results revealed that explant from seedling had higher regeneration and transformation rate under the condition of cultured 20 d in darkness first and then exposed 10 d in lighting environment. Acetosyringone (AS) concentration was a key factor impacting transformation rate, and the optimal performance of transformation was under 20 mg/L concentration of co-cultivated me