大鼠脑创伤后激活小胶质细胞表达MHC-II分子的-第三军医大学学报.DOC

脑创伤后大鼠血清作用下小胶质细胞MHC-Ⅱ的表达 马栋斌1，闫华2，苏心2，亢建民2（300070天津，天津医科大学研究生院1；300060天津，天津市环湖医院研究所2） [摘要]目的 观察大鼠脑创伤后，不同时间点的血清刺激体外培养的大鼠小胶质细胞后MHC-Ⅱ分子的表达。方法 采用改良Feeney自由落体法构建大鼠颅脑损伤模型，20只SD大鼠按随机数字表法分为对照组5只、假手术组5只和创伤组10只，创伤组按伤后时间分为2、4、8、16 h和24 h 5个采血点，在伤后不同时间点采用眼眶取血法，取静脉血离心后得血清刺激原代培养的大鼠小胶质细胞，培养24 h后流式细胞仪测定小胶质细胞MHC-Ⅱ的表达。结果 正常大鼠脑内小胶质细胞一般为静息状态，MHC-Ⅱ微量表达；假手术组有较低表达；创伤组各个时间点均有表达，且随着时间的推移，MHC-Ⅱ表达增高。创伤组与对照组两两比较，差异有统计学意义(P0.05)。结论 大鼠脑损伤后的血清可使小胶质细胞MHC-Ⅱ的表达增高，并具有时间相关性。 [关键词] 颅脑损伤；小胶质细胞；MHC-Ⅱ [通信作者] 亢建民，E-mail: Kangjianmin168@ Exploration of MHC-Ⅱ expression of microglia induced by cerebral trauma MA Dong-bin, YAN Hua, SU Xin, KANG Jian-min (Graduate School of Tianjin Medical University, Research Institution of Tianjin Huanhu Hospital ) [Abstract] Objective To observe the expression of MHC-Ⅱ of microglia in culture induced by cerebral trauma. Methods The craniocerebral injury model was created by modified Feeneys method, twenty healthy SD rats were randomly divided into three groups: normal group(5), sham(5), trauma group(10). The trauma group rats were drawn blood at 2h, 4h, 8h, 16h and 24h after injury through orbits. Get the serum after centrifuging to stimulate the microglia cultured in vitro. 24h later, MHC-Ⅱ expressed by microglia was measured by flow cytometry. Results In normal group, the microglia kept in a quiescent condition, and MHC-Ⅱ was weak or negative. The expression of MHC-Ⅱ in the trauma group were significantly higher than those in other two groups. Conclusions The serum after cerebral trauma can induce microglia expressing MHC-Ⅱ. [Key words] brain injury, microglia, MHC-Ⅱ Corresponding author: KANG Jian-min, Email: Kangjianmin168@中枢神经系统(central nervous system, CNS)因被血脑屏障(blood-brain barrier, BBB)保护、缺乏淋巴系统和免疫细胞而被称为“免疫豁免”区域，但脑内很多疾患的发生、发展中均伴有免疫反应[1]。 小胶质细胞（microglia，MG）在中枢神经系统分布较广，占胶质细胞的10%～20%，在CNS中起着支持、营养、保护及修复等重要功能，是神经系统中具有发表性的免疫细胞。作为中枢神经系统的常驻免疫细胞，小胶质细胞在脑组织对感染、外伤、疾病等免疫反应中，充当着主要媒介[2]。既往在神经退行性疾病和感染方面，对小胶质细胞的免疫功能研究较多。据报道，体外培养的小胶质细胞在革兰阴性细胞壁产物脂多糖(lipopolysaccharide)的作用下可以被激活而释放IL-