HPLC测定不同来源大黄中蒽醌和二蒽酮类成分.PDFVIP

·1114 · 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 6 期 2011 年 6 月 HPLC 测定不同来源大黄中蒽醌和二蒽酮类成分 王 哲，许利嘉，何春年，彭 勇，肖培根* 中国医学科学院 北京协和医学院药用植物研究所，北京 100193 摘 要：目的 建立同时测定大黄中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和番泻苷 A 、番泻苷 B 的 HPLC 法，并对不同来源的大黄样品进行测定。方法 采用 HPLC 梯度洗脱，色谱柱为 Agilent Eclipse XDB-C18 （150 mm×4.6 mm ， 5 μm ）；检测波长分别为254 nm 、340 nm；体积流量为 1 mL/min；柱温为30 ℃。结果 在一定范围内，芦荟大黄素、大黄 酸、大黄素、大黄酚、大黄素甲醚和番泻苷 A 、番泻苷 B 峰面积积分值与进样量线性关系良好，加样回收率符合要求，方 法具有较好的精密度、重现性和稳定性，可同时对多种来源大黄药材进行测定。结论 不同来源大黄的化学成分差异较大， 其中种植大黄样品中总蒽醌及总番泻苷的量均少于野生样品。 关键词：大黄；蒽醌；番泻苷；芦荟大黄素；大黄酸；大黄素；大黄酚；大黄素甲醚 中图分类号：R286.02 文献标志码：A 文章编号：0253 - 2670(2011)06 - 1114 - 05 Determination of anthraquinones and bianthrones in rhubarb from different sources by HPLC WANG Zhe, XU Li-jia, HE Chun-nian, PENG Yong, XIAO Pei-gen Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China Abstract: Objective To develop a HPLC method for determining the content of aloe-emodin, rhein, emodin, chrysophanol, physcion, sennoside A, and sennoside B simultaneously. The method was used in content determination of constituents in rhubarb from different sources. Methods The separations were carried out at 30 ℃ on an Agilent Eclipse XDB-C18 column (150 mm × 4.6 mm, 5μm) and eluted with acetonitril and water containing 0.1% phosphoric acid in gradient mode. The flow rate was 1.0 mL/min, detection wavelengths were 254 nm for aloe-emodin, rhein, emodin, chrysophanol, physcion and 340 nm for sennoside A and sennoside B, column temperature was 30 ℃. Results The peak areas and injection ammounts of aloe-emodin, rhein, emodin, chrysophanol, physcion, sennoside A and sennoside B showed a good linear relationship within a certain range. The average recoveries were standards-compliant.