酿酒酵母纤维素乙醇统合加工CBP的策略及研究进展.PDFVIP

生 物 工 程 学 报 Chin J Biotech 2010, July 25; 26(7): 870–879 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn ©2010 CJB, All rights reserved. 综 述 酿酒酵母纤维素乙醇统合加工 (CBP) 的策略及研究 进展 徐丽丽，沈煜，鲍晓明 山东大学 微生物技术国家重点实验室，济南 250100 摘 要: 木质纤维素乙醇的统合生物加工过程 (Consolidated bioprocessing ，CBP) 是将纤维素酶和半纤维素酶生产、纤维 素水解和乙醇发酵过程组合或部分组合，通过一种微生物完成。统合生物加工过程有利于降低生物转化过程的成本，越 来越受到研究者的普遍关注。酿酒酵母 Saccharomyces cerevisiae 是传统的乙醇发酵菌株。介绍了影响外源基因在酿酒酵 母中表达水平的因素，纤维素酶和半纤维素酶在酿酒酵母中表达研究进展及利用酿酒酵母统合加工纤维素乙醇的策略。 关键词: 木质纤维素，统合生物加工，纤维素酶系，纤维素乙醇，酿酒酵母 Progress and strategies on bioethanol production from lignocellulose by consolidated bioprocessing (CBP) using Saccharomyces cerevisiae Lili Xu, Yu Shen, and Xiaoming Bao State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China Abstract: Ethanol production from lignocelluloses of consolidated bioprocessing (CBP) is a system in which cellulase and hemicellulase production, substrate hydrolysis, and fermentation are combined or partially combined by ethanologen microorganisms that express cellulolytic or hemicellulolytic enzymes or engineering cellulolytic microorganisms with ethanol production properties. Due to its potential for significant cost reduction, CBP is receiving more and more attention. In this review article, we discuss the factors that influence the expression level of cellulases in Saccharomyces cerevisiae and updated progress in bioethanol production from lignocellulose by the CBP strategy using the yeast species. Keywords: lignocelluloses, consolidated bioprocessing, cellulases, lignocellulosic ethanol, Saccharomyces cerevisiae 新型能源的开发利用是当目前人类面对能源、