亚油酸对铜绿微囊藻的抑制机理.pdfVIP

中国环境科学 2009,29(4) ：419~424 China Environmental Science 亚油酸对铜绿微囊藻的抑制机理 张庭廷,郑春艳,何 梅,吴安平,聂刘旺* (安徽师范大学生命科学学院,生物环境与生态安全安徽省高校重点实 验室,安徽 芜湖 241000) 摘要：为有效利用化感物质控制水华藻类,实验探讨了亚油酸对铜绿微囊藻的抑制机理.结果表明,采用亚油酸处理铜绿微囊藻后,铜绿微囊 藻的叶绿素 a 含量下降、细胞外液中电导率、OD260 增加;细胞 O2 、MDA 产生增多; SOD 酶活力和比活力、谷胱甘肽-过氧化物酶(GSH-Px) 酶活力在前 4d 内应激性升高,SOD 同工酶的谱带在亚油酸浓度为 0.1mg/L 时表达增强.在亚油酸抑藻的实验组中同时加入抗氧化剂维生素 C 后, O2 的产生减少,藻生物量增加.因此推测亚油酸的抑藻机理可能是通过 O2 的产生,引起细胞质膜的膜质过氧化,造成细胞膜通透性 的改变,并导致 DNA 等生物大分子的损伤,从而抑制了叶绿素 a 以及有关蛋白质的合成,最终使藻细胞死亡与溶解. 关键词：亚油酸；铜绿微囊藻；抑制机理；超氧阴离子自由基 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2009)04-0419-06 The inhibitory mechanism of linoleic acid on Microcystis aeruginosa. ZHANG Ting-ting, ZHENG Chun-yan, HE * Mei, WU An-ping, NIE Liu-wang (The Key Laboratory of Biotic Environment and Ecological Safety in Anhui Province, College of Life Sciences, Anhui Normal University, Wuhu 241000, China). China Environmental Science, 2009,29(4) ：419~424 Abstract ：In order to effectively control water-bloom algae by using allelochemicals, the inhibitory mechanism of linoleic acid on M. aeruginosa was analyzed. After the linoleic acid added in the experimental groups, the chlorophyll a content of algal cells decreased, the electrical conductivity (EC) and the OD260 of algal extracellular fluid increased, the generation of malondialdehyde (MDA) and superoxide anion radical (O2 ) of the algal cells increased, activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) increased at the first 4 days and the expression of SOD isoenzyme enhanced at the concentration of linoleic acid being 0.1mg/L. But when antioxidants vitamin C was added the generation of O2 decreased, and algal biomass increased. All the results indicated that the mechanisms underlying the algal growth inhibition by linoleic acid involved the O2 generation that