棉仁植酸含量的离子色谱测定方法研究.pdfVIP

棉 花 学 报 Cotton Science 20 15袁27 渊6 冤院561―569 仁植酸含量的离子色谱测定方法研究 智辉 阎君 辛文慧 徐晓建 祝水金 陈进红* 浙江大学农业与生物技术学院农学系/ 江省作物种质资源重点实验室袁杭州310058 冤 摘要院为准确测定棉仁植酸含量袁本研究从提取工艺尧色谱条件改进等方面入手袁优化建立了棉仁植酸含量的 快速提取及离子色谱测定方法院棉仁粉样品烘干后用乙醚脱脂袁以10 mL 0.66 mol 窑L 1 的HCl 提取袁沸水浴 1 h袁4 ℃震荡 12 h 袁 离心分离袁 取上清液稀释并纯化后上样分析曰 采用DIONEX ICS-2000 离子色谱 尧 AG 11-HC Guard (4 mm 50 mm)保护柱和AG11-HC Analytical (4 mm 250 mm) 分离柱袁在30.0 mmol 窑L 1 的 KOH 为淋洗液袁流动相流速为 1.0 mL 窑min 1袁采样时间为25 min 袁抑制电流为75 mA 袁柱温30 ℃ 袁进样量20 2 滋L 的条件下进行检测遥 结果表明袁棉仁植酸的回归曲线 为0.999 袁在检测浓度内线性关系良好曰各项指标相 对标准偏差均在5% 以内袁平均回收率为98.64%～102.3 1%袁检出限 样品中检出待测物的最小浓度冤为0.059 滋g 窑mL 1袁定量限 样品中待测物能被定量测定的最低量冤为0.196 滋g 窑mL 1遥 关键词院棉仁曰植酸曰 离子色谱 中图分类号院O657.7+5 ∶S562 文献标志码院A 文章编号院1002-7807 渊20 15冤06-0561-09 10.11963/issn.1002-7807.20 1506008 Lu Zhihui, Yan Jun, Xin Wenhui, Xu Xiaoji an, Zhu Shuijin, Chen Jinhong* ( 310058, ) Cottonseed meal is an important plant protein resource of feed industry and breeding industry, although it contains many antinutritional factors. Phytic acid, as one of the mainly antinutritional factor, cannot be digested easily by monogastric an- imals, such as fowls and pigs, consequently results in the decreased bio-availability of material elements and leads to the envi- ronment pollution by the phytate in excreta. Thus far, study of breeding cottons containing low phytic acid gradually becomes the hotspot, and the method of fully extracting phytic acid from cotton seeds and precise measurement is becoming more and more important and necessary. A new method for the rapid extraction and d