棉花单核苷酸多态性标记研究进展.pdfVIP

棉 花 学 报 Cotton Science 20 16, 28(4): 399―406 棉花单核苷酸多态性标记研究进展 振玉 李威** 周晓箭 裴小雨 刘艳改 周克海 张文生 孟清芹 海风 葛勇 李莹 刘俊芳 马雄风* 杨代刚* 中国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455000 冤 摘要院单核苷酸多态性标记已在农作物研究中得到广泛应用并取得重大进展遥 为了便利棉花SNP Single nu- cleotide polymorphism冤标记的研究和应用袁介绍了利用基因芯片尧简化基因组测序尧重测序等在棉花中开发 SNP 标记的方法袁综述了SNP 标记在棉花遗传图谱构建尧数量位点的定位和分子标记辅助育种尧基因组测序 以及系统进化等研究中的应用遥 并对异源四倍 棉花 SNP 标记开发时袁同源序列位点和部分同源序列位点 上的SNP 标记辨别问题进行了系统探讨袁对其快捷的开发尧检测方式和在数量基因定位 的应用前景进行了 展望遥 关键词院棉花曰分子标记曰单核苷酸多态性曰表达序列标签曰基因组测序曰遗传图谱曰进展 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 20 16冤04-0399-08 10.11963/issn.1002-7807.20 1604012 Wang Zhenyu, Li Wei**, Zhou Xiaoji an, Pei Xiaoyu, Liu Yangai, Zhou Kehai, Zhang Wensheng, Meng * * Qingqin, Wang Haifeng, Ge Yong, Li Ying, Liu Junfang, Ma Xiongfeng , Yang Daigang ( 455000, ) Single nucleotide polymorphism (SNP) markers have been widely used in crop studies and significant progress has been made in recent years. In this article, we examine the development and research progress of SNP markers in cotton, includ- ing the methods of using gene chips, simplifying genome sequencing and resequencing to develop the SNP markers in cotton, and the applications of SNP markers in cotton such as genetic mapping, quantitative trait loci analysis, molecular marker-assisted breeding, genome sequencing, and phylogenetic analysis. The present problems in the development and application of SNP markers on homologous and homoeologous loci in allotetraploid cotton are also discussed. The anticipated prospects for SNP markers are also discussed in terms of rapid detec