张家口地区木糖氧化无色杆菌耐药性相关基因分析.docVIP

张家口地区木糖氧化无色杆菌耐药性相关基因分析了解张家口地区木糖氧化无色杆菌中耐药性相关基因的基因型别，为一定的方法 应用改良三维试验法筛选耐β-内酰胺类药物的菌，再结合技术和序列测定检测耐药菌ESBLs和AmpC酶的基因型别，进行统计学分析。结果 在2010年12月-2011年12月间本地区临床分离的株木糖无色杆菌菌株中有株单产ESBLs株单产AmpC酶株同时产ESBLs和AmpC酶株为菌株耐药基因型并发现存在多重耐药基因ESBLs以TEM型检出率最高，TEM-1亚型AmpC酶多重耐药基因TEM+CTX-M-1AmpC检出率最高。结论院木糖氧化无色杆菌耐药现象，临床应严格掌握头抱菌素的使用。关键词木糖氧化无色杆菌改良三维试验；超广谱β内酰胺酶；Analysis of drug resistance genes in Achromobacter xylosoxidans in Zhangjiakou WANG Weibao, WANG Jianfang, XIE Fei, NIU Shuli, WANG Zhixia (The fourth hospital of zhangjiakou, Zhangjiakou 075000, China ) ABSTRACT: Objective: To investigate the genetic type drug resistance genes in Achromobacter xylosoxidans in Zhangjiakou, and provide experimental basis for clinical use of antibiotics. Methods: Used Modified three-dimensional test to screen strain resistance to β-lactam drugs, then adhibited PCR and sequence analysis to test genetic type of ESBLs and AmpC. Results: Among 48 strains of Achromobacter xylosoxidans isolated in Zhangjiakou hospital from Dec 2010 to Dec 2011, 32 strains(66.7%) resistant to β-lactam drugs, 14 produced ESBLs, 5 produced AmpC, 9 produced both ESBLs and AmpC, 4 were unknown strain. 24 strain PCR results were positive, and belonged to different gene types. Multidrug resistance were found. TEM type was the highest ESBLs, and all were TEM-1 subtype; AmpC detection rate was higher, all belonged to DHA-1 type; TEM+CTX-M-1+AmpC was the highest Multidrug resistance type. Conclusions: The percentage of Achromobacter xylosoxidans resistance to β-lactam drugs were serious in hospitals of Zhangjiakou, there should strictly control the use of head embrace rhzomorph drugs in order to obtain more favorable curative effect. Key word: Achromobacter xylosoxidans; Modified three-dimensional test; Extended-spectrum β-lactamases; Cephalosporinase 木糖氧化无色杆菌是人类最常感染的无色杆菌菌种之一，随着β-内酰胺类抗菌药物的大量使用，菌的耐药性日益突出。本研究选取2010年12月-2011年12月间张家口地区各个医院分离到的木糖氧化无色杆菌作为研究对象，PCR技术和序列测定分析细菌中耐药性相关基因的基因型分布情况，为临一定的，报道如下。1 资料与方法 1.1 标本来源2010年12月-2011年12月间我市各医院门诊和住院患者中分离到的木糖