添加结球甘蓝4号染色体对不同基因型大白菜抽薹及BrFLCs基因表达的影响.pdfVIP

园艺学报，2015 ，42 (12) ：2405 –2411. Acta Horticulturae Sinica doi ：10.16420/j.issn.0513-353x.2015-0871；http ：//www. ahs. ac. cn 2405 添加结球甘蓝 4 号染色体对不同基因型大白菜 抽薹及BrFLCs基因表达的影响 顾爱侠，张雅琨，赵建军，王彦华，陈雪平，申书兴* （河北农业大学园艺学院，河北省蔬菜种质资源创新与利用重点实验室，河北保定071001 ） 摘 要：在获得耐抽薹大白菜—结球甘蓝 4 号染色体单体异附加系 AC4 的基础上，以其为母本分别 与不同基因型的 2 个耐抽薹大白菜自交系和 2 个易抽薹自交系杂交，利用根尖染色体计数法结合结球甘 蓝 4 号染色体相对于大白菜的特异分子标记鉴定，对杂交后代进行筛选，在每个杂交组合后代中分别获 得了 2 个 2n = 21 和 2 个 2n = 20 植株。抽薹现蕾时间鉴定结果表明每个杂交组合后代中 2n = 21 的植株均 较 2n = 20 的植株抽薹现蕾时间推迟。对杂交后代及其父本植株中BrFLCs 的表达进行了分析，结果表明 BrFLCs 基因表达量不是影响大白菜抽薹现蕾的唯一主要因素，基因组背景对抽薹现蕾时间效应大于 BrFLCs 基因，同时外源结球甘蓝 4 号染色体的添加对 BrFLCs 基因表达的效应受大白菜基因组背景的影 响。 关键词：大白菜；结球甘蓝；单体异附加系；抽薹；BrFLCs 中图分类号：S 634.1 文献标志码：A 文章编号：0513-353X （2015 ）12-2405-07 Effects of the Added Cabbage Chromosome 4 on Bolting Time and BrFLCs Expression of Chinese Cabbage * GU Ai-xia，ZHANG Ya-kun ，ZHAO Jian-jun ，WANG Yan-hua ，CHEN Xue-ping，and SHEN Shu-xing （College of Horticulture，Agricultural University of Hebei ，Key Laboratory of Vegetable Germplasm and Utilization of Hebei ，Baoding ，Hebei 071001 ，China ） Abstract ：Monosomic addition line of Chinese cabbage introduced alien cabbage chromosome 4 had been obtained. In this research ，the hybridizations between Chinese cabbage monosomic alien addition line and four Chinese cabbage inbred lines with early or late bolting were developed. These hybrids were identified by chromosome counting of root tip and chromosome 4 specific markers of cabbage compared with Chinese cabbage. Two 2n = 21 and two 2n = 20 plants were obtained in every cross combination. The results of bolting investigation for hybrids indicated that the bolting of plants with chromosome 2n = 21 were later than the plan