亚洲棉和雷蒙德氏棉MATE基因家族生物信息学及其同源基因在陆地棉中的表达分析.pdfVIP

棉 花学 报 Cotton Science 20 16, 28(3): 2 15―226 洲棉和雷蒙德氏棉MATE 基因家族生物信息学及其 同源基因在陆地棉中的表达分析 沈知临 许磊 陈文 吴楠 蔡永萍 林毅 高俊山* 安徽农业大学生命科学学院袁 徽合肥230036 冤 摘要院多药和有毒化合物排出(MATE)蛋白家族是1 个次级转运蛋白家族遥 为了更好地了解亚洲棉和雷蒙德氏 棉中MATE 蛋白的种类和数量袁 利用2 种二倍体棉花基因组的氨基酸和cDNA 数据库对MATE 基因家族进 行筛选袁并分析亚洲棉和雷蒙德氏棉全基因组中MATE 基因的种类和进化关系遥 结果显示袁在亚洲棉基因组 中初步鉴定了34 个MATE 基因袁而在雷蒙德氏棉中筛选出42 个MATE 基因遥基因结构和系统进化的比较分 析表明袁进化关系近的MATE 基因在结构上基本是一致的遥 同 对陆地棉中克隆的 12 及与其同一族的 部分MATE 基因进行荧光定量分析袁推测其所在同一族的MATE 基因功能可能与转运原花青素有关遥这些结 果为进一步深入分析棉花MATE 基因的功能以及在原花青素转运中的作用提供了理论基础遥 关键词院亚洲棉曰雷蒙德氏棉曰陆地棉曰MATE 基因家族曰基因结构曰系统进化分析曰荧光定量PCR 中图分类号院S562.03 文献标志码院A 文章编号院1002-7807 20 16冤03-0215-12 10.11963/issn.1002-7807.201603004 Shen Zhilin, Xu Lei, Chen Wen, Wu Nan, Cai Yongping, Lin Yi, Gao Junshan* ( 230036, ) The multidrug and toxic compound extrusion (MATE) protein belongs to a secondary transporter family. To better understand the variety and quantity of MATE protein in and , amino acid sequences and cDNA databases from two diploid cotton genomes were used to screen the MATE gene family, and to analyze species and evolutionary relationships of MATE genes. Thirty-four MATE genes were preliminarily identified in the genome of , and 42 in the genome. Comparative analyses of gene structure and phyletic evolution revealed the evo- lutionary relationship between MATE genes is consistent with structure. 12 from upland cotton , and other MATE genes from the same family, were analyzed by qRT-PCR. Results suggested the function of MATE gene members was likely related to the transfer of proanthocyanidin . These results provide a theoretical basis for further analysis of MATE gene function and its role in the tra