β-淀粉样蛋白脑室注射对大鼠海马星型胶质细胞-第三军医大学学报.docVIP

β-淀粉样蛋白脑室注射对大鼠海马星型胶质细胞活化和细胞增殖的作用 高长越( 1,唐军2，王延江1，许志强1，邓娟1，周华东( ( 1 (1第三军医大学大坪医院神经内科 400042) (2第三军医大学生理学教研室 　 400038) 摘要 目的　通过观察大鼠侧脑室注射β-淀粉蛋白（Aβ）后海马GFAP的表达，探讨Aβ对海马局部星型胶质细胞活化增生的作用及对海马神经发生的可能影响。方法　将Aβ1-42注射入大鼠侧脑室，分别于术后3天，7天，14天，30天处死动物，应用免疫组化方法观察GFAP的表达，并用免疫荧光双标标记Brdu、GFAP以判断增殖细胞的种类。结果　实验组大鼠海马GFAP阳性细胞明显增加,到7天达到高峰,21天后逐渐下降，较对照组差异显著，实验组各时相点Brdu、GFAP双标结果显示有部分Brdu阳性细胞GFAP为阳性， 对照组双标阳性细胞很少。结论　β-淀粉蛋白脑室注射后大鼠海马星型胶质细胞活化增生，部分增生的星型胶质细胞可能来源于神经干细胞。 关键词 β-淀粉样蛋白;海马;星型胶质细胞;AD Astrocyte activation and proliferation in rat hippocampus after unilateral i.c.v. of Aβ Gao Changyue,Tang Jun ,Wang Yanjiang, Xu Zhiqiang,Deng Juan, Zhou Huadong Department of Neurology ,Daping Hospital,Third Military Medical University, 400042 Department of Physiology , Third Military Medical University, 400038 Abstract Objective The expression of GFAP in hippocampus was detected to observe the astrocyte activation and neurogenesis of the area after unilateral intracerebroventricular injection（i.c.v.） of β-amyloid peptide(Aβ).Methods Adult male SD rats were divided into control group and experimental group randomly.Experimental group were infused with Aβ1-42 into the lateral ventricle and sacrificed 3d,7d,14d,30d after infusion. GFAP was abserved by immunohistochemistry .The double labelling of Brdu and GFAP was abserved by immunofluorescence.Results The GFAP positive cells increased significantly after injection and reached maximal expression on the 7th day，and decreased slowly after 21st day.. Some of Brdu positive cells were GFAP positive,while there are few double positive cells in the control group. Conclusion : Astrocytes are activated after i.c.v. injection of Aβ,and part of activated astrocytes come from the NSCs in hippocampus. Aβ may be one of the factors promoting NSCs to develop into astrocytes. Key words: Aβ; hippocampus; astrocyte; AD (((阿尔茨海默病(Alzheimer Disease，AD)是一种中枢神经系统退行性疾病，在世界范围内有近两千万患者，严重威胁着人类健康。其特征为临床上进行性的痴呆和β-淀粉蛋白(β-amyloid peptide, Aβ)在脑内病理性沉积，后者目前被认为是AD发病的中心环节[1]。海马是AD最易受累脑区，而海马齿状回下