鱼肉中氯霉素检测用抗体包被金磁纳米微粒修饰安培-传感技术学报.pdfVIP

第 22 卷 　第 10 期 传 感 技 术 学 报 Vol . 22 　N o . 10 2009 年 10 月 CHIN ES E J OU RNAL O F S EN SORS AND AC TUA TORS Oct . 2009 Amperous Immunosensor f or Chlora mphenicol in Fish Based on Antibody Coated Gol dMagnetic Nano Particles Modif ied Electrode X I E D ong h ua1 , 2 , GA N N i ng 1 , WA N G Feng 1 , YA N G X i n 1 1 T he S t ate Key L aboratory B ase of N ovel F unct ional M ateri als an d P rep arat ion S cience , N i ng bo Uni vers ity , N i ng bo Z hej i ang 3 152 11 , Chi na; 2 N i ng bo Ent ry E x i t I nsp ect ion an d Quarant i ne B u reau of t he Peop les R ep ubl ic of Chi na , N i ng bo Z hej i ang 3 15200 , Chi na Abstract :A magnetic nano p article s mo dified amp erou s immuno sen sor for rapid sep arationf ree det er mina tion of chloramp henicol ( CA P) in fi sh i s p ropo sed . The immuno sen sor i s p rep ared by coimmobilizin g CA P antibo dy (anti CA P) coat ed Fe O / A u magnetic nanop article s ( GM P) and t riet hylenet et ramine copp er ( II) 3 4 ( CuL ) on a screen p rint ed p yro genation grap hit e elect ro de ( P GE) t hrough adding magnetic fiel d ab sorp tion ( PR G| CuL/ anti CA PGM P) . CuL can be emp loyed a s an elect roreductive cat alyzation for H O Aft er 2 2 t he immuno sen sor i s incubat ed wit h CA P solution at 25 ℃for 30 min ,t he elect ron acce ss bet ween acti