积雪草酸对多发性骨髓瘤细胞增殖活性的影响及其机制探讨.pdfVIP

14 4 中草药 Chinese Traditional and Herbal Drugs 41 9 2010 9 * 吕婷婷, 刘 芳 , 陈 燕, 姜旭东 ( , 430022) : RPM I 8226 M T T ; Ho echest3325 8 ; ( FCM ) ; RT- P CR sur v v n bcl- 2 m RN A 10 ~ 70 mol/ L RPM I 8226 , , 244872 h I C50 ( 4225 457) ( 2488 35 1) ( 1983 288) mo l/ L , , 25~ 40 mo l/ L RPM I 8226 G , G surv v n bc-l 2 m RN A 2 2 , RPM I 8226 , surv v n bc-l 2 : ; ; R PM I 8226; ; surv v n ; bc-l 2 : R2 855 : A : 0253- 2670( 20 10) 09- 1484- 05 Effect of asiatic acid on proliferation of multiple myeloma cells and its mechanism L T ng- t ng , LIU F ang, CH EN Y an, J IA N G Xu- dong ( Inst tute of H emat olo g y, U n o n H osp tal , T ongj M ed cal Colleg e, H uazhong U n v ers ty of Sc ence and T echnolog y, Wuhan 4 30022 , Ch na) Abstract: Objective T o explore t he ef fect o f as at c ac d on cell pro l frerat o n, apopt os s, and cells cycle of human mult ple m yelom a cells RP MI 8226, and ts ant tumo r m echan sm . Methods M T T A ssay w as u sed t o assess t he ef f ect of as at c ac d on cells prol f erat on ; H oechest 3325 8 st a n ng w as st ud ed t o ev aluat e the morpholog cal chang es n RP M I 8226 cells nduced by as at c ac d ; F low cyt om et ry w as used t o analyz e t he nduct on apo pt os s and cell cycle ar rest of as at c ac d; RT- P CR w as appl ed t o det ect t he chan- g es of surv v n and bc-l 2 m RNA ex press on lev els . Results A s at c ac d s g n f cantly nh b t ed t he cell pro- l f erat on o f RPM I 8226 n mol/ L gr ade level, n a t m e- and dose- dependent manner . T he IC50 v alues of RPM I 8226 cells w ere ( 42