响应面法优化发芽豇豆积累γ-氨基丁酸工艺条件的研究 optimization of gaba accumulation during cowpea germination by response surface methodology.pdfVIP
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响应面法优化发芽豇豆积累γ-氨基丁酸工艺条件的研究 optimization of gaba accumulation during cowpea germination by response surface methodology
10 2010,V01.31,No.02 食品科学 ※工艺技术
响应面法优化发芽豇豆积累y一氨基丁酸
工艺条件的研究
申迎宾,范子剑,麻浩事
(南京农业大学作物遗传与种质创新国家重点实验室,大豆研究所,江苏南京 210095)
摘 要:以豇豆为原料,利用发芽过程积累P.氨基丁酸。通过单因素及响应面试验研究浸泡温度和时间、发芽
温度和时间等因素对卜氨基丁酸积累量的影响,确定豇豆最佳发芽工艺参数。结果表明:在浸泡温度34C和浸泡
中’,.氨基丁酸的7.48倍。同时建立浸泡温度和时间,发芽温度和时间与卜氨基丁酸含量之间的二次多项式模型,
该模型可以很好地预测豇豆在发芽过程中的’,.氨基丁酸含量。
关键词:豇豆;发芽;r一氨基丁酸富集;优化;响应面
ofGABAAccumulation Germination Surface
by
Optimization duringCowpea ResponseMethodology
SHEN Hao。
Ying-bin,FANZi-jian,MA
of Geneticsand
(StateKeyLaboratoryCrop CjermplasmEnhancement,Soybean黜黼hInstitute,NangjingAgriculturalUniversity,
210095,China)
Nanjins
to conditionsfor
Abstract:In柚attemptoptimize伽啊p∞germinationgamma-aminobutyric
as
individualeffectsof andtime勰well andtimeonGABAaccumulationin
soakingtemperature germinationtemperature
factormethodand central
wa=e on
germinatedcowpeainvesdgatedbysingle subsequently,basedcompositedesign,interactive
effectsof Weleevaluated surface conditionsfor
the
germinationparameters byresponsemethodology.Theoptimum cowpea
Weleobtainedas time25 33℃and
germination follows:soakingtemperature34℃。soakingh,germinationtemperature
time the in
24h.Under GABAcontent reached203.53
germination optimalconditions,the germinatedcowpea mg/100g,which
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