伪狂犬病毒变异株_JS_2012_对仔猪的致病性研究.pdfVIP

中国动物传染病学报 2014,22(5): 10-14 Chinese Journal of Animal Infectious Diseases ·研究论文· 伪狂犬病毒变异株（JS-2012 ）对仔猪的致病性研究 童 武，郑 浩，单同领，刘 飞，梁 超，李国新，姜一峰，于 海，高 飞，周艳君，童光志 （中国农业科学院上海兽医研究所，上海200241 ） 摘 要：本实验室2012年从江苏省太仓市某发病猪场分离到1株变异的伪狂犬病毒，命名为JS-2012 。为了研究该分离株对仔猪的 致病性，将12头15 日龄的伪狂犬病毒阴性仔猪随机分为3组，其中2组（每组5头猪）分别通过肌肉注射和滴鼻接种JS-2012毒， 第3组2 头猪做空白对照。接种病毒的仔猪在攻毒后24 h体温均开始升高，4 d后开始死亡，死亡率为100％。临床观察发现，滴 鼻组仔猪发病死亡明显快于肌肉注射组。对病死猪进行剖解，均可见大脑血管扩张并出血等典型的伪狂犬病病理变化。病理切 片显示猪的脑蛛网膜下腔严重出血，其他各脏器也均有严重病理变化。结果表明该变异株JS-2012为伪狂犬强毒毒株。 关键词：伪狂犬病毒；JS-2012 ；仔猪；致病性 中图分类号：S852.659.1 文献标志码：A 文章编号：1674-6422 （2014 ）05-0010-05 PATHOGENICITY OF VARIANT STRAIN JS-2012 OF PSEUDORABIES VIRUS IN PIGLETS TONG Wu, ZHENG Hao, SHAN Tong-ling, LIU Fei, LIANG Chao, LI Guo-xin, JIANG Yi-feng, YU Hai, GAO Fei, ZHOU Yan-jun, TONG Guang-zhi (Shanghai Veterinary Research Institute, CAAS, Shanghai 200241, China) Abstract: In December 2012, a variant strain JS-2012 of porcine Pseudorabies virus (PRV) was isolated from a diseased piglet in Taicang city Jiangsu Province. To study the pathogenicity of JS-2012, twelve 15-day-old PRV-free piglets were divided into 3 groups: intramuscular injection group (5 piglets), intranasal inoculation group (5 piglets) and non-treatment control group (2 piglets). At 24 h post infection, body temperature of piglets in ten 15-day-old inoculation piglets in two infection groups increased. At day 4 post infection, piglets in both infected group started to die. The intranasal group died faster than those in intramuscular injection group although the mortality of both infection groups was 100%. Dead piglets were necropsied for pathological examination. Vasodilatation and angiorhagia were found in brain, which were typical lesions caused by PRV. Severe subarachnoid hemorrhage and lesion