仿生膜内固定的多巴胺对抗坏血酸的氧化作用-分析化学.PDFVIP

29 ( FENXI HUAXUE) 8 20018 C inese Journal of Analytical C emistry 881~ 884 吴正岩 唐纪琳 汪尔康* ( 中国科学院长春应用化学研究所电分析化学开放研究实验室, 国家电化学和光谱研究分析中心, 长春 130022) , 2. 0 10- 3 mol L , 260 mV , , , 1 1 10 , Kunitake , , , , , 2 , 3 4 20 90 , Rusling Nakas ima , , Rusling , 5 6 P450cam , , , ( C) , , 7 8 9, 10 , , , , , - ( DMPC) , , , 260 mV, , , , 2 2. 1 Cypress System CS- 1087 ( Kansas T. Kuwana ) , , ( 4 mm) , AgAgCl , 2. 2 Sigma , 3 ( pH 7. 0) 0. 005 molL NaH2 PO4 , 0. 005 molL Na2HPO4 0. 1 molL NaCl, , Millipore Q ( Millipore ) ( 18. 2 Mcm) 2000-09-18; 2000-12-09 882 29 2. 3 1. 0 m0. 3 m 0. 05 m Al2 O3 , 1 min DMPC , 2 gL, 2 L , , , 30 min , DMPC 3 3. 1 DMPC 1 DMPC , , Ep 500 mV, , 5 Rusling Nassar , , 1 DMPC 0.01 , molL , Fig. 1 Cyclic voltammogram of a glassy carbon ( GC) DMPC electrode cast wit dimyristoylp osp atidylc oline DMPC 10 ( DMPC) film in 001molL dopamine solution , ( pH= 7. 0) ( buffer ) : 5 mmolL 2 (p osp ate buffer) ( pH 7. 0) ( scan rate) : 20 mVs 2a DMPC 2 DMPC ( a) (b) Fig. 2 Cyclic voltammogram of dopamine in cast DMPC film ( a) and relations ip between peak current of dopamine in cast film and square root of scan rat