不同理化因子对枇杷(Eriobotrya japonica(Thunb) L)花粉体外萌发和花粉管生长的影响.pdfVIP

Botanical Research 植物学研究, 2014, 3, 164-171 Published Online July 2014 in Hans. /journal/br /10.12677/br.2014.34021 Effects of the Different Factors on Pollen Germination and Pollen Tube Growth in Eriobotrya japonica (Thunb.) L. Jiao Zhou, Lingming Ge, Xin Hu, Xingchun Tang* School of Life and Science, Hubei University, Wuhan * Email: tangxingchun@ th th th Received: Apr. 25 , 2014; revised: May 20 , 2014; accepted: May 28 , 2014 Copyright © 2014 by authors and Hans Publishers Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). /licenses/by/4.0/ Abstract In this study, loquat fresh pollens were used as materials to explore the effect of sucrose concen- tration, pH and gamma-amino butyric acid (GABA) on pollen germination and pollen tube growth in vitro. The basic culture medium consists of 31 mg/L boric acid + 196.8 mg/L MgSO ·7H O + 7.45 4 2 mg/L KCl + 56 mg/L CaCl2. Pollens were cultured in dark at 25°C for 5 h. The results showed that pollen germination rate reached to 60% and pollen tube growth rate was 70.4 µm/h. Given 10% sucrose and pH value 8.0, pollen germination reached the highest ratio of 63%. Moreover, the ad- dition of GABA to the medium can effectively promote the growth rate of pollen tubes. Keywords Eriobotrya japonica (Thunb.) L., Pollen Germination, Pollen Tube Growth, GABA 不同理化因子对枇杷(Eriobotrya japonica (Thunb.) L.)花粉体外萌发和花粉管生长的影响 周 娇，葛凌明，胡 欣，汤行春* 湖北大学生命科学学院，武汉 * Email: tangxingchun@ *通讯作者。 164 不同理化因子对枇杷(Eriobotrya japo